A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.

Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio Hideo; Santoro, Marcelo Matos; Andrade, Milton Hércules Guerra de

Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/jspui/handle/123456789/4576

Título:	A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.
Autor(es):	Santana, Marcos Aurélio de Santos, Alexandre Martins Costa Oliveira, Marcelo Eurípedes Oliveira, Jamil Silvano de Babá, Élio Hideo Santoro, Marcelo Matos Andrade, Milton Hércules Guerra de
Palavras-chave:	Macrotyloma axillare Lectin Purification Antigen
Data do documento:	2008
Referência:	SANTANA, M. A. de et al. A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin. International Journal of Biological Macromolecules, v. 43, p. 352-358, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0141813008001578>. Acesso em: 08 nov. 2014.
Resumo:	The N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.
URI:	http://www.repositorio.ufop.br/handle/123456789/4576
DOI:	https://doi.org/10.1016/j.ijbiomac.2008.07.011
ISSN:	0141-8130
Licença:	O periódico International Journal of Biological Macromolecules concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3546550273168.
Aparece nas coleções:	DECBI - Artigos publicados em periódicos

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