A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.

Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio Hideo; Santoro, Marcelo Matos; Andrade, Milton Hércules Guerra de

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Campo Dublin Core	Valor	Idioma
dc.contributor.author	Santana, Marcos Aurélio de	-
dc.contributor.author	Santos, Alexandre Martins Costa	-
dc.contributor.author	Oliveira, Marcelo Eurípedes	-
dc.contributor.author	Oliveira, Jamil Silvano de	-
dc.contributor.author	Babá, Élio Hideo	-
dc.contributor.author	Santoro, Marcelo Matos	-
dc.contributor.author	Andrade, Milton Hércules Guerra de	-
dc.date.accessioned	2015-03-10T13:56:43Z	-
dc.date.available	2015-03-10T13:56:43Z	-
dc.date.issued	2008	-
dc.identifier.citation	SANTANA, M. A. de et al. A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin. International Journal of Biological Macromolecules, v. 43, p. 352-358, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0141813008001578>. Acesso em: 08 nov. 2014.	pt_BR
dc.identifier.issn	0141-8130	-
dc.identifier.uri	http://www.repositorio.ufop.br/handle/123456789/4576	-
dc.description.abstract	The N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.	pt_BR
dc.language.iso	en_US	pt_BR
dc.subject	Macrotyloma axillare	pt_BR
dc.subject	Lectin	pt_BR
dc.subject	Purification	pt_BR
dc.subject	Antigen	pt_BR
dc.title	A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.	pt_BR
dc.type	Artigo publicado em periodico	pt_BR
dc.rights.license	O periódico International Journal of Biological Macromolecules concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3546550273168.	pt_BR
dc.identifier.doi	https://doi.org/10.1016/j.ijbiomac.2008.07.011	-
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