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Título: | Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) biochemical characterization, immunolocalization and possible role in host cell adhesion. |
Autor(es): | Moura, Christiane Mariotini Bastos, Matheus Silva e Castro, Felipe Freitas de Trindade, Mellina Lanna Vasconcellos, Raphael de Souza Vale, Myriam Augusta Araújo Neves do Moreira, Bernardo Pereira Santos, Ramon de Freitas Oliveira, Claudia Miranda de Cunha, Luana Celina Seraphim Souto, Xênia Macedo Bressan, Gustavo Costa Silva Júnior, Abelardo Baqui, Munira Muhammad Abdel Bahia, Maria Terezinha Almeida, Márcia Rogéria de Fernandes, José Roberto Meyer Fietto, Juliana Lopes Rangel |
Palavras-chave: | Recombinant protein |
Data do documento: | 2014 |
Referência: | MOURA, C. M. et al. Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) biochemical characterization, immunolocalization and possible role in host cell adhesion. Acta Tropica, v. 130, p. 140-147, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X13003318>. Acesso em: 08 nov. 2014. |
Resumo: | Previous work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localiza- tion and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of triphosphate and diphosphate nucleosides. The enzyme’s Km for ATP (0.096mM) suggested a capabil-ity to influence the host’s ATP-dependent purinergic signaling. The use of specific polyclonal antibodies allowed us to confirm the presence of TcNTPDase-1 at the surface of parasites by confocal and electron microscopy. In addition, electron microscopy revealed that TcNTPDase-1 was also found in the flagellum, flagellum insertion region, kinetoplast, nucleus and intracellular vesicles. The presence of this enzyme in the flagellum insertion region and vesicles suggests that it may have a role in nutrient acquisition, and the widespread distribution of TcNTPDase-1 within the parasite suggests that it may be involved in other biological process. Adhesion assays using anti-TcNTPDase-1 polyclonal antibodies as a blocker or purified recombinant TcNTPDase-1 as a competitor revealed that the enzyme has a role in parasite–host cell adhesion. These data open new frontiers to future studies on this specific parasite–host interaction and other unknown functions of TcNTPDase-1 related to its ubiquitous localization. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4931 |
DOI: | https://doi.org/10.1016/j.actatropica.2013.11.008 |
ISSN: | 0001-706X |
Licença: | This article is published under the terms of the Creative Commons Attribution-NonCommercial-No Derivatives License (CC BY NC ND). Fonte: o próprio artigo. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_TrypanossomaCruziNucleoside.pdf | 1,62 MB | Adobe PDF | Visualizar/Abrir |
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