Anti–zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. leaves.
Nenhuma Miniatura disponível
Data
2023
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the
Brazilian Cerrado, whose ethanolic extract showed significant in vitro anti–Zika virus activity by the
MTT colorimetric method. Currently, there is no drug in clinical use specifically for the treatment
of this virus; therefore, in this work, the antiviral and cytotoxic properties of the ethanolic extract,
fractions, and compounds were evaluated. The ethanolic extract of the leaves showed no cytotoxicity
for the human MRC-5 cell and was moderately cytotoxic for the Vero cell (CC50 161.5 ± 2.01 μg/mL).
This extract inhibited the Zika virus multiplication cycle with an EC50 of 85.2 ± 1.65 μg/mL. This
extract was fractionated using the liquid–liquid partition technique, and the ethyl acetate fraction
showed significant activity against the Zika virus with an EC50 of 40.7 ± 2.33 μg/mL. From the
ethyl acetate fraction, the flavonoids quercetin-3-O-hexosylgallate (1), quercetin-3-O-glucoside (2),
and quercetin (5) were isolated, and in addition to these compounds, a mixture of quercetin-3-
O-rhamnoside (3) and quercetin-3-O-arabinoside (4) was also obtained. The isolated compounds
quercetin and quercetin-3-O-hexosylgallate inhibited the viral cytopathic effect at an EC50 of 18.6
± 2.8 and 152.8 ± 2.0, respectively. Additionally, analyses by liquid chromatography coupled to a
mass spectrometer allowed the identification of another 24 minor phenolic constituents present in the
ethanolic extract and in the ethyl acetate fraction of this species.
Descrição
Palavras-chave
Cytotoxicity, Phytochemistry
Citação
LIMA, L. D. A. et al. Anti–zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. leaves. Molecules, v. 28, n. 6, artigo 2546, 2023. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0378517323005677>. Acesso em: 01 ago. 2023.