Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease.
dc.contributor.author | Menezes, Cristiane Alves da Silva | |
dc.contributor.author | Sullivan, A. K. | |
dc.contributor.author | Falta, M. T. | |
dc.contributor.author | Mack, D. G. | |
dc.contributor.author | Freed, B. M. | |
dc.contributor.author | Rocha, Manoel Otávio da Costa | |
dc.contributor.author | Gollob, Kenneth John | |
dc.contributor.author | Fontenot, A. P. | |
dc.contributor.author | Dutra, Walderez Ornelas | |
dc.date.accessioned | 2017-06-05T16:40:42Z | |
dc.date.available | 2017-06-05T16:40:42Z | |
dc.date.issued | 2012 | |
dc.description.abstract | Human infection with Trypanosoma cruzi leads to Chagas disease, which presents as several different clinical conditions ranging from an asymptomatic form to a severe dilated cardiomyopathy. Several studies have demonstrated that T cells play a critical role in the development of cardiac pathology, as well as in immunoregulation during chronic disease. However, the mechanisms that drive protective or pathogenic T cell response are not known.We have shown that CD4+ T cells from chagasic patients preferentially express T cell receptor (TCR) b-chain variable region (Vb) 5. The aim of this work was to determine whether T cells expressing this particular Vb region displayed variable or restricted CDR3 sequences, as an indicator of the nature of the stimulus leading to the activation of these T cells in vivo. Additionally, we aimed to evaluate phenotypic characteristics of these cells that might be associated with pathology. CDR3 junctional region sequencing of Vb5·1 expressing CD4+ T cells revealed the occurrence of a highly homologous CDR3 region with conserved TCR Jb region usage among patients with cardiac, but not indeterminate, Chagas disease. Moreover, correlation analysis indicated that the frequency of CD4+Vb5·1+ cells is associated with granzyme A expression, suggesting that these cells might display cytotoxic function. Together these results provide new insight into T cell recognition of antigens involved in Chagas disease and suggest that these cells may be implicated in the pathogenesis of chagasic cardiomyopathy. | pt_BR |
dc.identifier.citation | MENEZES, C. A. da S. et al. Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease. Clinical and Experimental Immunology, v. 169, p. 109-118, 2012. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2012.04608.x/abstract>. Acesso em: 19 fev. 2017. | pt_BR |
dc.identifier.doi | https://doi.org/10.1111/j.1365-2249.2012.04608.x | |
dc.identifier.issn | 1365-2249 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/7880 | |
dc.identifier.uri2 | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2012.04608.x/abstract | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.title | Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |