Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease.
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2012
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Human infection with Trypanosoma cruzi leads to Chagas disease, which
presents as several different clinical conditions ranging from an asymptomatic
form to a severe dilated cardiomyopathy. Several studies have demonstrated
that T cells play a critical role in the development of cardiac pathology,
as well as in immunoregulation during chronic disease. However, the mechanisms
that drive protective or pathogenic T cell response are not known.We
have shown that CD4+ T cells from chagasic patients preferentially express T
cell receptor (TCR) b-chain variable region (Vb) 5. The aim of this work was
to determine whether T cells expressing this particular Vb region displayed
variable or restricted CDR3 sequences, as an indicator of the nature of the
stimulus leading to the activation of these T cells in vivo. Additionally, we
aimed to evaluate phenotypic characteristics of these cells that might be associated
with pathology. CDR3 junctional region sequencing of Vb5·1 expressing
CD4+ T cells revealed the occurrence of a highly homologous CDR3 region
with conserved TCR Jb region usage among patients with cardiac, but not
indeterminate, Chagas disease. Moreover, correlation analysis indicated that
the frequency of CD4+Vb5·1+ cells is associated with granzyme A expression,
suggesting that these cells might display cytotoxic function. Together these
results provide new insight into T cell recognition of antigens involved in
Chagas disease and suggest that these cells may be implicated in the pathogenesis
of chagasic cardiomyopathy.
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MENEZES, C. A. da S. et al. Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease. Clinical and Experimental Immunology, v. 169, p. 109-118, 2012. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2012.04608.x/abstract>. Acesso em: 19 fev. 2017.