Adhesion on yeast cell surface as a trapping mechanism of pathogenic bacteria by Saccharomyces probiotics.
Nenhuma Miniatura disponível
Data
2012
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Recently, much attention has been given to the use of probiotics as an adjuvant for the
prevention or treatment of gastrointestinal pathology. The great advantage of therapy with
probiotics is that they have few side effects such as selection of resistant bacteria or
disturbance of the intestinal microbiota, which occur when antibiotics are used. Adhesion of
pathogenic bacteria onto the surface of probiotics instead of onto intestinal receptors could
explain part of the probiotic effect. Thus, this study evaluated the adhesion of pathogenic
bacteria onto the cell wall of Saccharomyces boulardii and Saccharomyces cerevisiae strains
UFMG 905, W303 and BY4741. To understand the mechanism of adhesion of pathogens to
yeast, cell-wall mutants of the parental strain of Saccharomyces cerevisiae BY4741 were used
because of the difficulty of mutating polyploid yeast, as is the case for Saccharomyces
cerevisiae and Saccharomyces boulardii. The tests of adhesion showed that, among 11
enteropathogenic bacteria tested, only Escherichia coli, Salmonella Typhimurium and
Salmonella Typhi adhered to the surface of Saccharomyces boulardii, Saccharomyces
cerevisiae UFMG 905 and Saccharomyces cerevisiae BY4741. The presence of mannose, and
to some extent bile salts, inhibited this adhesion, which was not dependent on yeast viability.
Among 44 cell-wall mutants of Saccharomyces cerevisiae BY4741, five lost the ability to fix the
bacteria. Electron microscopy showed that the phenomenon of yeast–bacteria adhesion
occurred both in vitro and in vivo (in the digestive tract of dixenic mice). In conclusion, some
pathogenic bacteria were captured on the surface of Saccharomyces boulardii,
Saccharomyces cerevisiae UFMG 905 and Saccharomyces cerevisiae BY4741, thus
preventing their adhesion to specific receptors on the intestinal epithelium and their subsequent
invasion of the host.
Descrição
Palavras-chave
Citação
TIAGO, F. da C. P. et al. Adhesion on yeast cell surface as a trapping mechanism of pathogenic bacteria by Saccharomyces probiotics. Journal of Medical Microbiology, v. 61, p. 1194-1207, 2012. Disponível em: <http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.042283-0#tab2> . Acesso em: 16 jun. 2017.