Development and validation of an analytical method for quantification of arsenic and antimony in liposomes using inductively coupled plasma-optical emission spectrometry.
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2013
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Arsenic and antimony compounds are used to treat
endemic diseases, such as cancer, leishmaniasis,
and schistosomiasis, in spite of their toxicity.
Several studies seeking the development and
characterization of nanocarrier systems such
as liposomes are being carried out with the aim
of developing new drug delivery systems and
minimizing the toxicity of these drugs. However,
the lack of reference methods to quantify these
semimetals within a liposomal matrix hinders the
QC of these formulations. Therefore, the validation
of an analytical method for arsenic and antimony
quantification in liposomal matrix by inductively
coupled plasma-optical emission spectrometry is
presented here. The linearity, specificity, LOD, LOQ,
accuracy, and precision were determined according
to the International Conference on Harmonization
norms and the Brazilian Health Surveillance Agency
(Resolution 899). The LOD values were 0.02 and
0.06 mg/L for antimony and arsenic, respectively.
The LOQ for both was 3.0 mg/L, with an adequate
accuracy within 98.26 and 101.32% for different
levels of antimony and 99.98 and 100.36% for
arsenic. Precision (CV) was lower than 5.0%. The
developed and validated method was shown to
be reproducible for quantification of arsenic and
antimony in liposome pharmaceutical dosage forms.
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REIS, P. G. dos et al. Development and validation of an analytical method for quantification of arsenic and antimony in liposomes using inductively coupled plasma-optical emission spectrometry. Journal of AOAC International, v. 96, p. 771-775, 2013. Disponível em: <http://aoac.publisher.ingentaconnect.com/contentone/aoac/jaoac/2013/00000096/00000004/art00018>. Acesso em: 06 out. 2017.