Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.
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1993
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Cells of the yeast Saccharomyces cerevisiae display a
wide range of glucose-induced regulatory phenomena,
including glucose-induced activation of the
RAS-adenylate cyclase pathway and phosphatidylinosrtot
turnover, rapid post-translatronal effects on the
activity of different enzymes as well as long-term
effects at the transcriptional level. A gene called GGS1
(for general Glucose Sensor) that is apparently
required for the glucose-induced regulatory effects
and several ggsi aHeles (fic/pf, bypi and cifi) has been
cloned and characterized. A GGS1 homologue is
present in Methanobacterium thermoautotrophicum.
Yeast ggsi mutants are unable to grow on glucose or
Received 25 November, 1992; revised and accepted 15 February, 1993.
•For correspondence. Tel. (16) 220931; Fax (16) 204415. tThese two
authors contributed equally to this paper.
related readily fermentable sugars, apparently owing
to unrestricted influx of sugar Into glycolysis, resulting
in its rapid deregulation. Levels of intracellular free
glucose and metabolites measured over a period of a
few minutes after addition of glucose to cells of a
ggsi^ strain are consistent with our previous suggestion
of a functional interaction between a sugar
transporter, a sugar kinase and the GGS1 gene product.
Such a glucose-sensing system might both
restrict the influx of glucose and activate several
signal transduction pathways, leading to the wide
range of glucose-induced regulatory phenomena. Deregulation
of these pathways in ggsi mutants might
explain phenotypic defects observed in the absence of
glucose, e.g. the inability of ggsi diploids to sporulate.
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AELST, L. V et al. Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae. Molecular Microbiology, v. 82, p. 927-943, 1993. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1993.tb01638.x/epdf>. Acesso em: 10 jan. 2017.