Relationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modifed nanocapsules.

dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.contributor.authorLegrand, Philippe
dc.contributor.authorGulik, Annette
dc.contributor.authorBourdon, Olivier
dc.contributor.authorGref, Ruxandra
dc.contributor.authorLabarre, Denis
dc.contributor.authorBarratt, Gillian
dc.date.accessioned2014-11-10T17:11:54Z
dc.date.available2014-11-10T17:11:54Z
dc.date.issued2001
dc.description.abstractThe aim of our work was to examine the relationship between modi"cations of the surface of nanocapsules (NC) by adsorption or covalent grafting of poly(ethylene oxide) (PEG), and changes in their phospholipid (PL) content on complement activation (C3 cleavage) and on uptake by macrophages. The physicochemical characterization of the NC included an investigation of their properties, such as surface charge, size, hydrophilicity, morphology and homogeneity. This is the "rst time that such properties have been correlated with biological interactions for NC, a novel carrier system with a structure more complex than nanospheres. C3 crossed immunoelectrophoresis revealed the reduced activation for NC with longer PEG chain and higher density, although all formulations induced C3 cleavage to a lesser or greater extent. NC bearing PEG covalently bound to the surface were weaker activators of complement than plain PLA [poly(D,L-lactide)] NC or nanospheres (NS). Furthermore, the #uorescent/confocal microscopy of J774A1 cells in contact with NC reveal a dramatically reduced interaction with PEG-bearing NC. However, the way in which PEG was attached (covalent or adsorbed) seemed to a!ect the mechanism of uptake. Taken together, these results suggest that the low level of protein binding to NC covered with a high density of 20 kDa PEG chains is likely to be due to the steric barriers surrounding these particles, which prevents protein adsorption and reduces their interaction with macrophages. 2001 Elsevier Science Ltd. All rights reserved.pt_BR
dc.identifier.citationMOSQUEIRA, V. C. F. et al. Relationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modifed nanocapsules. Biomaterials, Guildford, v. 22, n. 22, p. 2967-2979, 2001. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0142961201000436>. Acesso em: 20 ago. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/S0142-9612(01)00043-6
dc.identifier.issn0142-9612
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/3753
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Biomaterials concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460320954961.pt_BR
dc.subjectNanocapsulespt_BR
dc.subjectPoly(D,L-lactide-co-ethylene oxide) copolymerspt_BR
dc.subjectComplement activationpt_BR
dc.subjectCellular uptakept_BR
dc.subjectPhysicochemical characterizationpt_BR
dc.titleRelationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modifed nanocapsules.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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