Cyclic AMP and low molecular weight effector(s) present in yeast extract are involved in pectin lyase production by Penicillium griseoroseum cultured on glucose.

dc.contributor.authorPereira, Maria Cristina Baracat
dc.contributor.authorCoelho, Jorge Luiz Cavalcante
dc.contributor.authorMinussi, Rosana Cristina
dc.contributor.authorAlves, Virgínia Maria Chaves
dc.contributor.authorBrandão, Rogélio Lopes
dc.contributor.authorSilva, Daison Olzany
dc.date.accessioned2017-03-22T16:36:49Z
dc.date.available2017-03-22T16:36:49Z
dc.date.issued1999
dc.description.abstractPectin lyase (PL) induction by organic and inorganic components of yeast extract (YE) was evaluated in Penicillium griseoroseum, cultured in a mineral medium containing sucrose, by determining PL activity (A235) and mycelial growth (mycelial dry weight). The lowest YE concentration that promoted significant PL induction without acting as a carbon source for the fungus corresponded to 0.0075%. Neither calcined YE nor a nutrient solution containing micronutrients induced PL production, indicating that the inducer was an organic compound. Vitamins, phospholipid components, amino acids, and nitrogenous bases were tested in place of YE and promoted no significant PL induction. A PL inducer compound was found to be soluble in the nucleotide fraction obtained during extraction of YE. The inducer was shown to be a thermostable polar substance dialyzable at 2000 Daltons, hydrolyzable by HCl, and activated by boiling for up to 60 min. Cyclic AMP (cAMP) exogenously added to the culture medium at 5 and 10 mM was capable of inducing PL in P. griseoroseum grown on sucrose, suggesting that at least one compound may be present in YE acting in a cooperative fashion for the maintenance of high levels of cAMP into the cell. PL activity and the level of cAMP inside the fungal cells increased after the addition of YE to the culture medium, suggesting the participation of this messenger in this enzyme’s synthesis.pt_BR
dc.identifier.citationPEREIRA, M. C. B. et al. Cyclic AMP and low molecular weight effector(s) present in yeast extract are involved in pectin lyase production by Penicillium griseoroseum cultured on glucose. Applied Biochemistry and Biotechnology, v. 76, p. 129-141, 1999. Disponível em: <http://link.springer.com/article/10.1385/ABAB%3A76%3A2%3A129>. Acesso em: 10 jan. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1385/ABAB:76:2:129
dc.identifier.issn1559-0291
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7424
dc.identifier.uri2http://link.springer.com/article/10.1385/ABAB%3A76%3A2%3A129pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectPectinasespt_BR
dc.titleCyclic AMP and low molecular weight effector(s) present in yeast extract are involved in pectin lyase production by Penicillium griseoroseum cultured on glucose.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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