Evaluation of EPS extraction protocols from anaerobic sludge for gel-based proteomic studies.
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Data
2015
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Despite the importance of anaerobic sludge extracellular polymeric substances (EPSs), their
characterization is limited to information regarding their chemical classes and molecular size. This
work explores the possibility of using proteomic techniques to study the proteins present in this
matrix. Thus, this paper compares eight EPS extraction methods regarding extraction yield, protein/
carbohydrate ratio, size distribution profile and suitability to sodium dodecyl sulfate–polyacrylamide
gel electrophoresis analyses. Despite the differences found in quantification and size exclusion
chromatography assays, the band profile found for all methods was very similar. Considering the
band pattern, extraction time and background level, heating method followed by ammonium sulfate
precipitation proved to be the most appropriate method for gel-based analyses of anaerobic sludge
EPS proteins.
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Anaerobic digestion, EPS proteins
Citação
ZOREL, J. A. et al. Evaluation of EPS extraction protocols from anaerobic sludge for gel-based proteomic studies. Water Science and Technology, v. 72, p. 535, 2015. Disponível em: <http://iwaponline.com/content/72/4/535>. Acesso em: 05 ago. 2017.