Oral formulation angiotensin-(1-7) therapy attenuates pulmonary and systemics damage in mice with emphysema induced by elastase.

dc.contributor.authorBastos, Aline Cândida
dc.contributor.authorMagalhães, Giselle Santos
dc.contributor.authorGregório, Juliana Fabiana
dc.contributor.authorMatos, Natália Alves de
dc.contributor.authorSantos, Daisy Motta
dc.contributor.authorBezerra, Frank Silva
dc.contributor.authorSantos, Robson Augusto Souza dos
dc.contributor.authorSantos, Maria José Campagnole dos
dc.contributor.authorMachado, Maria da Glória Rodrigues
dc.date.accessioned2021-11-22T11:49:04Z
dc.date.available2021-11-22T11:49:04Z
dc.date.issued2020pt_BR
dc.description.abstractAngiotensin-(1-7) [Ang-(1-7)], a peptide of the renin-angiotensin system, has anti-inflammatory, anti-fibrotic and antiproliferative effects in acute or chronic inflammatory disease of respiratory system. In this study, we evaluated the effect of treatment with Ang-(1-7) on pulmonary tissue damage and behavior of mice submitted to experimental model of elastase-induced pulmonary emphysema (PE). Initially, male C57BL/6 mice were randomly assigned into two main groups: control (CTRL) and PE. In the PE group, the animals received three intratracheal instillations of pancreatic porcine elastase (PPE) at 1-week intervals (0.2 IU in 50 μL of saline). The CTRL group received the same volume of saline solution (50 μL). Twenty-four hours after the last instillation, animals of the PE group were randomly divided into two groups: PE and PE + Ang-(1-7). The PE + Ang-(1-7) group was treated with 60 μg/kg of Ang-(1-7) and 92 μg kg of HPβCD in gavage distilled water, 100 μl. The CTRL and PE groups were treated with vehicle (HPβCD- 92 μg/kg in distilled water per gavage, 100 μl), orally daily for 3 weeks. On the 19th day of treatment, all groups were tested in relation to locomotor activity and exploratory behavior. After 48 h, the animals were euthanized and lungs were collected. The animals of PE group presented rupture of alveolar walls and consequently reduction of alveolar tissue area. Treatment with Ang-(1-7) partially restored the alveolar tissue area. The PE reduced the locomotor activity and the exploratory behavior of the mice in relation to the control group. Treatment with Ang-(1-7) attenuated this change. In addition, it was observed that Ang-(1-7) reduced lung levels of IL-1β and increased levels of IL-10. These results show an anti-inflammatory effect of Ang-(1-7), inducing the return of pulmonary homeostasis and attenuation of the behavioral changes in experimental model of PE by elastase.pt_BR
dc.identifier.citationBASTOS, A. C. et al. Oral formulation angiotensin-(1-7) therapy attenuates pulmonary and systemics damage in mice with emphysema induced by elastase. Immunobiology, v. 225, p. 151893, 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S0171298519302086?via%3Dihub>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.imbio.2019.12.002pt_BR
dc.identifier.issn0171-2985
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13981
dc.identifier.uri2https://www.sciencedirect.com/science/article/abs/pii/S0171298519302086?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectMurine modelpt_BR
dc.subjectRenin-angiotensin systempt_BR
dc.subjectMAS receptorpt_BR
dc.titleOral formulation angiotensin-(1-7) therapy attenuates pulmonary and systemics damage in mice with emphysema induced by elastase.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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