Adenosine actions are preserved in corpus cavernosum from obese and type II diabetic db/db mouse.
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2008
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Introduction—Erectile dysfunction (ED) in diabetes is associated with autonomic neuropathy
and endothelial dysfunction. Whereas the nonadrenergic-noncholinergic (NANC)/neurogenic
nitric oxide pathway has received great attention in diabetes-associated ED, few studies have
addressed sympathetic overactivity.
Aim—To test the hypothesis that adenosine-induced inhibition of adrenergic-mediated contractile
responses in mouse corpus cavernosum is impaired in the presence of diabetes.
Methods—The db/db (obesity and type II diabetes caused by a leptin receptor mutation) mouse
strain was used as a model of obesity and type II diabetes, and standard procedures were
performed to evaluate functional cavernosal responses.
Main Outcome Measures—Increased cavernosal responses to sympathetic stimulation in db/
db mice are not associated with impaired prejunctional actions of adenosine.
Results—Electrical field stimulation (EFS)-, but not phenylephrine (PE)-, induced contractions
are enhanced in cavernosal strips from db/db mice in comparison with those from lean littermates.
Direct effects of adenosine, 2-chloro-adenosine, A1 receptor agonist C-8031 (N6
cyclopentyladenosine), and sodium nitroprusside are similar between the strips from lean and db/
db mice, whereas relaxant responses to acetylcholine and NANC stimulation are significantly
impaired in the cavernosal strips from db/db mice. 5′-Iodotubercidin (adenosine kinase inhibitor)
and dipyridamole (inhibitor of adenosine transport), as well as the A1 agonist C-8031,
significantly and similarly inhibit contractions induced by stimulation of adrenergic nerves in the
cavernosal strips from lean and db/db mice.
Conclusions—Results from this study suggest that corpora cavernosa from obese and diabetic
db/db mice display altered neural-mediated responses that would favor penile detumescence, i.e.,
increased contractile response to adrenergic nerve stimulation and decreased relaxant responses
upon activation of NANC nerves. However, increased cavernosal responses to adrenergic nerve
stimulation are not due to impaired negative modulation of sympathetic neurotransmission by
adenosine in this diabetic model.
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Obesity, Diabetes, Sympathetic Neurotransmission, Animal Models
Citação
CARNEIRO, F. S. et al. Adenosine actions are preserved in corpus cavernosum from obese and type II diabetic db/db mouse. The Journal of Sexual Medicine, v. 5, p. 1156-1166, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1743609515320385>. Acesso em: 10 jan. 2017.