In vitro and in vivo acute toxicity of a novel citrate-coated magnetite nanoparticle.
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2022
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Magnetic nanoparticles (MNps) have become powerful tools for multiple biomedical applications such as hyperthermia drivers, magnetic resonance imaging (MRI) vectors, as well as
drug-delivery systems. However, their toxic effects on human health have not yet been fully
elucidated, especially in view of their great diversity of surface modifications and functionalizations. Citrate-coating of MNps often results in increased hydrophilicity, which may positively impact their performance as drug-delivery systems. Nonetheless, the consequences
on the intrinsic toxicity of such MNps are unpredictable. Herein, novel magnetite (Fe3O4)
nanoparticles covered with citrate were synthesized and their potential intrinsic acute toxic
effects were investigated using in vitro and in vivo models. The proposed synthetic pathway
turned out to be simple, quick, inexpensive, and reproducible. Concerning toxicity risk
assessment, these citrate-coated iron oxide nanoparticles (IONps) did not affect the in vitro
viability of different cell lines (HaCaT and HepG2). Moreover, the in vivo acute dose assay
(OECD test guideline #425) showed no alterations in clinical parameters, relevant biochemical variables, or morphological aspects of vital organs (such as brain, liver, lung and kidney).
Iron concentrations were slightly increased in the liver, as shown by Graphite Furnace
Atomic Absorption Spectrometry and Perls Prussian Blue Staining assays, but this finding
was considered non-adverse, given the absence of accompanying functional/clinical repercussions. In conclusion, this study reports on the development of a simple, fast and reproducible method to obtain citrate-coated IONps with promising safety features, which may be
used as a drug nanodelivery system in the short run.
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Alanine aminotransferase, Alkaline phosphatase
Citação
ROCHA, J. M. V. et al. In vitro and in vivo acute toxicity of a novel citrate-coated magnetite nanoparticle. PLoS One, v. 17, artigo e0277396, 2022. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0277396>. Acesso em: 01 ago. 2023.