High-density-immune-complex regulatory macrophages promote recovery of experimental colitis in mice.
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2021
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Resumo
Macrophages not only play a fundamental role in the pathogenesis of inflam-
matory bowel disease (IBD), but they also play a major role in preserving intestinal homeo-
stasis. In this work, we evaluated the role of macrophages in IBD and investigated whether the
functional reprogramming of macrophages to a very specific phenotype could decrease dis-
ease pathogenesis. Thus, macrophages were stimulated in the presence of high-density
immune complexes which strongly upregulate their production of IL-10 and downregulate
pro-inflammatory cytokines. The transfer of these high-density-immune-complex regulatory
macrophages into mice with colitis was examined as a potential therapy proposal to control
the disease. Animals subjected to colitis induction received these high-density-immune-
complex regulatory macrophages, and then the Disease Activity Index (DAI), and macro-
scopic and microscopic lesions were measured. The treated group showed a dramatic
improvement in all parameters analyzed, with no difference with the control group. The
colon was macroscopically normal in appearance and size, and microscopically colon architecture was preserved. The immunofluorescence migration assay showed that these cells
migrated to the inflamed intestine, being able to locally produce the cytokine IL-10, which
could explain the dramatic improvement in the clinical and pathological condition of the
animals. Thus, our results demonstrate that the polarization of macrophages to a high IL-10
producer profile after stimulation with high-density immune complexes was decisive in
controlling experimental colitis, and that macrophages are a potential therapeutic target to
be explored in the control of colitis.
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Inflammatory bowel diseases, Ulcerative colitis
Citação
LOPES, T. C. M. et al. High-density-immune-complex regulatory macrophages promote recovery of experimental colitis in mice. Inflammation, v. 44, p. 1069–1082, 2021. Disponível em: <https://link.springer.com/article/10.1007/s10753-020-01403-w#citeas>. Acesso em: 11 out. 2022.