Influence of normal microbiota on some aspects of the immune response during experimental infection with Trypanosoma cruzi in mice.
Nenhuma Miniatura disponível
Data
2004
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
To study the influence of normal associated microbiota on systemic immunological responses
during experimental Chagas’ disease, germ-free and conventional NIH Swiss mice were infected
with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia
were found, conventional mice showed IFN-ª, TNF-Æ and NO production (P , 0.05) by spleen cell
cultures and higher blood levels of immunoglobulins of the IgG2a isotype (P , 0.05) when
compared to their germ-free counterparts. Moreover, higher levels of IgG1 were also found in
conventional animals. On the other hand, no differences in IL10 production were found between
germ-free and conventional mice after infection (P , 0.05). Interestingly, spleen cell cultures from
non-infected germ-free mice spontaneously produced higher levels of IL10 than cultures from
conventional mice. Moreover, cultures from non-infected germ-free mice responded to T. cruzi
antigens with IFN-ª production, contrary to cultures from conventional animals. In conclusion, the
presence of the normal microbiota skews the immune response towards production of inflammatory
cytokines during experimental infection with T. cruzi in mice. However, the increase in production of
cytokines that is linked to resistance to this parasite did not alter the outcome of infection
significantly, probably due to high virulence of the Y strain.
Descrição
Palavras-chave
Citação
DUARTE, R. et al. Influence of normal microbiota on some aspects of the immune response during experimental infection with Trypanosoma cruzi in mice. Journal of Medical Microbiology, v. 53, p. 741-748, 2004. Disponível em: <http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45657-0#tab2>. Acesso em: 22 fev. 2017.