Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with leishmania chagasi.
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2011
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Nitric oxide (NO), the product of the nitric oxide
synthase enzymes has been detected in Leishmania-infected
animals. Besides its role on the immunity to infection, the
role of NO and the inducible nitric oxide synthase (iNOS)
in the pathogenesis of canine visceral leishmaniasis (CVL)
is not well understood. This study aimed at evaluating
immunohistochemically the iNOS expression in the spleen
of dogs naturally infected (ID) with Leishmania (L.)
chagasi compared with non-infected dogs (NID). The ID
was grouped according to the clinical form and the parasite
load. Symptomatic dogs (SD) presented higher parasite
load in relation to oligosymptomatic (OD) and asymptomatic
(AD). The qualitative expression of iNOS was
observed only in ID. SD presented strong and prominent
labeling of iNOS, followed by OD and AD. Quantitatively,
the results showed that the median expression of iNOS was
higher in SD and OD compared to NID. Also, dog spleens
with high parasitism load showed marked iNOS expression.
Taken together, the results suggest that the expression of
iNOS in the spleen of infected dogs with CVL was
associated with clinical worsening of the disease and with
high parasitism.
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SANTOS, F. R. et al. Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with leishmania chagasi. Parasitology Research, v. 108, p.1397-1403, 2001. Disponível em: <http://link.springer.com/article/10.1007/s00436-010-2183-5>. Acesso em: 10 out. 2016.