A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum.
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2022
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Vaccination against visceral leishmaniasis (VL) should be considered as a control measure to protect against
disease, and amastigote-specific proteins could help to develop such vaccines, since this parasite form is in
contact with the host immune system during the active disease. In this study, a Leishmania amastigote-specific
protein, LiHyG, was evaluated as recombinant protein (rLiHyG) as vaccine candidate against Leishmania infan-
tum infection in BALB/c mice. The protein was associated with saponin (rLiHyG/Sap) or Poloxamer 407-based
polymeric micelles (rLiHyG/Mic) as adjuvants, and animals receiving saline, saponin or micelle as controls.
Immunological and parasitological analyses were performed before (n = 8 per group; as primary endpoint) and
after (n = 8 per group; as secondary endpoint) infection. Results showed that, in both endpoints, rLiHyG/Sap and
rLiHyG/Mic induced higher levels of IFN-γ, IL-12 and GM-CSF in spleen cell cultures from vaccinated animals,
besides elevated presence of IgG2a isotype antibodies. Decreased hepatotoxicity and ‘positive lymphoprolifer-
ative response were also found after challenge. Such findings reflected in significantly lower levels of parasite
load found in their spleens, livers, bone marrows and draining lymph nodes. In conclusion, rLiHyG associated
with Th1-type adjuvant could be considered for future studies as vaccine candidate to protect against VL.
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Polymeric micelles, Saponin, Hypothetical protein, Amastigote, Visceral leishmaniasis
Citação
MACHADO, A. S. et al. A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum. Acta tropica, v. 230, artigo 106412, jun. 2022. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X22001103?via%3Dihub>. Acesso em: 11 out. 2022.