LC UV methodology for simultaneous.
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Data
2008
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Resumo
This study describes an accurate, sensitive, and specific chromatographic method for the
simultaneous quantitative determination of lamivudine and zidovudine in human blood
plasma, using stavudine as an internal standard. The chromatographic separation was
performed using a C8 column (150 9 4.6 mm, 5 lm), and ultraviolet absorbency detection
at 270 nm with gradient elution. Two mobile phases were used. Phase A contained 10 mM
potassium phosphate and 3% acetonitrile, whereas Phase B contained methanol. A linear
gradient was used with a variability of A-B phase proportion from 98–2% to 72–28%,
respectively. The drug extraction was performed with two 4 mL aliquots of ethyl acetate.
Descrição
Palavras-chave
Column liquid chromatography, Lamivudine, Zidovudine, Stavudine
Citação
SOUZA, J de et al. LC UV methodology for simultaneous. Chromatographia, Wiesbaden, v. 69, p. S231-S235, 2008. Disponível em: <http://link.springer.com/article/10.1365/s10337-008-0913-y> Acesso em: 10 jan. 2017