Up-regulation of SUMO E3 ligases during lung schistosomula and adult worm stages.
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2014
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Resumo
Small ubiquitin-like modifier (SUMO) conjugation
of proteins occurs through a concert action of enzymes
using a similar ubiquitination mechanism. After a
C-terminal peptide is cleaved from the SUMO precursor
by a protease to reveal a di-glycine motif, SUMO is
activated by an E1 enzyme (Aos1/Uba2) and conjugated
to target proteins by the sole E2 enzyme (Ubc9) guided to
the appropriate substrates by the SUMO E3 ligase. Previous
reports from our group showed that Schistosoma
mansoni has two paralogs of SUMO: one E2 conjugation
Ubc9 and two SUMO-specific proteases (SENPs). The
differential gene expression profile observed for SUMO
pathway genes throughout the S. mansoni life cycle attests
for the distinct patterns of SUMO conjugates observed
during parasite development particularly during the cercariae
to schistosomula transition. To continue this investigation,
we here analysed the repertoire of SUMO E3
ligases and their expression profiles during cercariae/
schistosomula transition. In silico analysis through
S. mansoni databases showed two conserved SUMO E3
ligases: protein inhibitor of activated STAT (PIAS) and
Ran-binding protein 2 (RanBP2). Furthermore, expression
levels of the SUMO E3 ligases were measured by
qRT-PCR using total RNA from cercariae, adult worms
and mechanically transformed schistosomula. Our data
showed an up-regulation of expression in lung
schistosomula and adult worm stages. In conclusion, the
differential expression of SmPIAS and SmRanBP2 during
schistosomula development was similar to the expression
levels of all genes related to SUMO conjugation, thereby
suggesting that the control of protein activity, localisation
or stability during cercariae to schistosomula transition is
SUMO-dependent.
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Schistosomula development, Differential expression
Citação
PEREIRA, R. V. et al. Up-regulation of SUMO E3 ligases during lung schistosomula and adult worm stages. Parasitology Research , v. 01, p. 1-7, 2014. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-014-3841-9>. Acesso em: 23 fev. 2017.