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Título: | DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum. |
Autor(es): | Carneiro, Fernando Silva Vitorino, Fernanda Regina Casagrande Giachini Lima, Victor Vitorino Carneiro, Zidonia N. Nunes, Kenia Pedrosa Ergul, Adviye Leite, Romulo Passaglia, Rita de Cassia Aleixo Tostes Webb, Robert Clinton |
Palavras-chave: | Rho-kinase Atrasentan |
Data do documento: | 2008 |
Referência: | CARNEIRO, F. S. et al. DOCA-salt treatment enhances responses to endothelin-1 in murine corpus cavernosum. Canadian Journal of Physiology and Pharmacology, v. 86, p. 320-328, 2008. Disponível em: <https://www.nrcresearchpress.com/doi/10.1139/Y08-031#.Xclvj1VKjm4>. Acesso em: 10 jan. 2017. |
Resumo: | The penis is kept in the flaccid state mainly via a tonic activity of norepinephrine and endothelins (ETs). ET-1 is important in salt-sensitive forms of hypertension. We hypothesized that cavernosal responses to ET-1 are enhanced in deoxycorticosterone acetate (DOCA)-salt mice and that blockade of ETA receptors prevents abnormal responses of the corpus cavernosum in DOCA-salt hypertension. Male C57BL/6 mice were unilaterally nephrectomized and treated for 5 weeks with both DOCA and water containing 1% NaCl and 0.2% KCl. Control mice were uninephrectomized and received tap water with no added salt. Animals received either the ETA antagonist atrasentan (5 mg·day−1·kg−1 body weight) or vehicle. DOCA-salt mice displayed increased systolic blood pressure (SBP), and treatment with atrasentan decreased SBP in DOCA-salt mice. Contractile responses in cavernosal strips from DOCA-salt mice were enhanced by ET-1, phenylephrine, and electrical field stimulation (EFS) of adrenergic nerves, whereas relaxations were not altered by IRL-1620 (an ETB agonist), acetylcholine, sodium nitroprusside, and EFS of nonadrenergic noncholinergic nerves. PD59089 (an ERK1/2 inhibitor), but not Y-27632 (a Rho-kinase inhibitor), abolished enhanced contractions to ET-1 in cavernosum from DOCA-salt mice. Treatment of DOCA-salt mice with atrasentan did not normalize cavernosal responses. In summary, DOCA-salt treatment in mice enhances cavernosal reactivity to contractile, but not to relaxant, stimuli, via ET-1/ETA receptor-independent mechanisms. |
URI: | http://www.repositorio.ufop.br/handle/123456789/7562 |
Link para o artigo: | https://www.nrcresearchpress.com/doi/10.1139/Y08-031#.Xclvj1VKjm4 |
DOI: | https://dx.doi.org/10.1139%2FY08-031 |
ISSN: | 1205-7541 |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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ARTIGO_DOCASaltTreatment.pdf Restricted Access | 411,37 kB | Adobe PDF | Visualizar/Abrir |
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