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Campo Dublin Core | Valor | Idioma |
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dc.contributor.author | Brandão, Rogélio Lopes | - |
dc.contributor.author | Castro, Ieso de Miranda | - |
dc.contributor.author | Bambirra, Eduardo Alves | - |
dc.contributor.author | Amaral, Sheila Coutinho | - |
dc.contributor.author | Fietto, Luciano Gomes | - |
dc.contributor.author | Trópia, Maria José Magalhães | - |
dc.contributor.author | Neves, Maria José | - |
dc.contributor.author | Santos, Raquel Gouvêa dos | - |
dc.contributor.author | Gomes, Newton Carlos Marcial | - |
dc.contributor.author | Nicoli, Jacques Robert | - |
dc.date.accessioned | 2017-03-20T12:55:44Z | - |
dc.date.available | 2017-03-20T12:55:44Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | BRANDÃO, R. L. et al. Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae. Applied and Environmental Microbiology, v. 642, p. 564-568, 1998. Disponível em: <http://aem.asm.org/content/64/2/564.long> Acesso em: 10 jan. 2017 | pt_BR |
dc.identifier.issn | 1098-5336 | - |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/7394 | - |
dc.description.abstract | As is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts. | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.title | Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
dc.identifier.uri2 | http://aem.asm.org/content/64/2/564.long | pt_BR |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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