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Title: Trypanosoma cruzi antigens induce inflammatory angiogenesis in a mouse subcutaneous sponge model.
Authors: Silva, Francisca Hildemagna Guedes da
Bajracharya, Deena Shrestha
Salles, Beatriz Cristina Silveira
Figueiredo, Vivian Paulino
Lopes, Laís Roquete
Dias, Luiza
Barcelos, Luciola da Silva
Moura, Sandra Aparecida Lima de
Andrade, Silvia Passos de
Silva, André Talvani Pedrosa da
Keywords: Trypanosoma cruzi
Sponge model
Inflammatory angiogenesis
Issue Date: 2015
Citation: SILVA, F. H. G. da et al. Trypanosoma cruzi antigens induce inflammatory angiogenesis in a mouse subcutaneous sponge model. Microvascular Research, v. 97, p. 130-136, 2015. Disponível em: <>. Acesso em: 15 out. 2015.
Abstract: Acute inflammation and angiogenesis are persistent features of several pathological conditions induced by biological agents leading to the resolution of local and systemic events. Glycoproteins derived from the protozoan Trypanosoma cruzi are suggested to mediate angiogenesis induced by inflammatory cells with still undescribed mechanisms. In this study, we investigated the effects of total antigen from trypomastigote forms of T. cruzi (Y strain), inoculated in sponges 24 h after implantation inmice, on angiogenesis, inflammatory cell pattern and endogenous production of inflammatory and angiogenicmediators on days 1, 4, 7 and 14 post-implant. There was an increase in hemoglobin content and in the number of blood vessels associated with T. cruzi antigen stimuli on the 14th day, assessed by the hemoglobin of the implants and by morphometric analysis. However, these antigens were not able to increase type I collagen content on the 14th day. Parasite antigens also induced high production of vascular endothelial growth factor (VEGF) and inflammatory mediators TNF-alpha, CCL2 and CCL5 on the 7th day in sponges when compared to the unstimulated group. Neutrophils and macrophages were determined by measuring myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAG) enzyme activities, respectively. Only NAG was increased after stimulation with antigens, starting from day 4 and peaking at day 7. Together, these data showed that antigens fromthe Y strain of T. cruzi are able to promote inflammatory neovascularization probably induced by macrophage-induced angiogenic mediators in T. cruzi antigen-stimulated sponges in Swiss mice.
ISSN: 0026-2862
metadata.dc.rights.license: O periódico Microvascular Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3732991075265.
Appears in Collections:DECBI - Artigos publicados em periódicos

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