Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4610
Title: Baclofen into the lateral parabrachial nucleus induces hypertonic sodium chloride intake during cell dehydration.
Authors: Kimura, Everton Heidi
Oliveira, Lisandra Brandino de
Menani, José Vanderlei
Callera, João Carlos
Keywords: Baclofen
Dehydration
Sodium appetite
Thirst
Lateral parabrachial nucleus
Issue Date: 2013
Citation: KIMURA, E. H. et al. Baclofen into the lateral parabrachial nucleus induces hypertonic sodium chloride intake during cell dehydration. Behavioral and Brain Functions, v. 9, p. 17, 2013. Disponível em: <http://www.behavioralandbrainfunctions.com/content/9/1/17>. Acesso em: 08 nov. 2014.
Abstract: Background: Activation of GABAB receptors with baclofen into the lateral parabrachial nucleus (LPBN) induces ingestion of water and 0.3 M NaCl in fluid replete rats. However, up to now, no study has investigated the effects of baclofen injected alone or combined with GABAB receptor antagonist into the LPBN on water and 0.3 M NaCl intake in rats with increased plasma osmolarity (rats treated with an intragastric load of 2 M NaCl). Male Wistar rats with stainless steel cannulas implanted bilaterally into the LPBN were used. Results: In fluid replete rats, baclofen (0.5 nmol/0.2 μl), bilaterally injected into the LPBN, induced ingestion of 0.3 M NaCl (14.3 ± 4.1 vs. saline: 0.2 ± 0.2 ml/210 min) and water (7.1 ± 2.9 vs. saline: 0.6 ± 0.5 ml/210 min). In cell-dehydrated rats, bilateral injections of baclofen (0.5 and 1.0 nmol/0.2 μl) into the LPBN induced an increase of 0.3 M NaCl intake (15.6 ± 5.7 and 21.5 ± 3.5 ml/210 min, respectively, vs. saline: 1.7 ± 0.8 ml/210 min) and an early inhibition of water intake (3.5 ± 1.4 and 6.7 ± 2.1 ml/150 min, respectively, vs. saline: 9.2 ± 1.4 ml/150 min). The pretreatment of the LPBN with 2-hydroxysaclofen (GABAB antagonist, 5 nmol/0.2 μl) potentiated the effect of baclofen on 0.3 M NaCl intake in the first 90 min of test and did not modify the inhibition of water intake induced by baclofen in cell-dehydrated rats. Baclofen injected into the LPBN did not affect blood pressure and heart rate. Conclusions: Thus, injection of baclofen into the LPBN in cell-dehydrated rats induced ingestion of 0.3 M NaCl and inhibition of water intake, suggesting that even in a hyperosmotic situation, the blockade of LPBN inhibitory mechanisms with baclofen is enough to drive rats to drink hypertonic NaCl, an effect independent of changes in blood pressure.
URI: http://www.repositorio.ufop.br/handle/123456789/4610
ISSN: 17449081
metadata.dc.rights.license: Authors of articles published in Behavioral and Brain Functions are the copyright holders of their articles and have granted to any third party, in advance and in perpetuity, the right to use, reproduce or disseminate the article. Fonte: Authors of articles published in BMC Research Notes are the copyright holders of their articles and have granted to any third party, in advance and in perpetuity, the right to use, reproduce or disseminate the article. Fonte: Behavioral and Brain Functions . Acesso em 05 dez. 2014.
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