Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/3779
Title: American tegumentary leishmaniasis : effectiveness of an immunohistochemical protocol for the detection of leishmania in skin.
Authors: Alves, Cibele Fontes
Alves, Cíntia Fontes
Figueiredo, Maria Marta
Souza, Carolina Carvalho de
Coelho, George Luiz Lins Machado
Melo, Maria Norma
Tafuri, Washington Luiz
Raso, Pedro
Soares, Rodrigo Pedro Pinto
Tafuri, Wagner Luiz
Issue Date: 2013
Citation: ALVES, C. F. et al. American tegumentary leishmaniasis : effectiveness of an immunohistochemical protocol for the detection of leishmania in skin. Plos One, v. 08, p. e63343, 2013. Disponível em: <http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0063343&representation=PDF>. Acesso em: 01 set. 2014.
Abstract: Background: American tegumentary leishmaniasis (ATL) is endemic in Latin America, where Brazil has over 27 thousand cases per year. The aim of the present study was to develop an immunohistochemical method (IHC) for ATL diagnosis. For this purpose, we used serum from a dog naturally infected with Leishmania (L) infantum (canine hyperimmune serum) as the primary antibody, followed by a detection system with a secondary biotinylated antibody. Methodology: Skin samples were obtained from 73 patients in an endemic area of Caratinga, Minas Gerais (MG) State, Brazil all testing positive for ATL with the Montenegro skin test, microscopy, and PCR. Canine hyperimmune serum of a dog naturally infected with Leishmania (L.) infantum was employed as a primary antibody in an immunohistochemical diagnostic method using streptavidin-biotin peroxidase. To assess the specificity of this reaction, IHC assays employing two monoclonal antibodies were carried out. As the polymer-based technology is less time-consuming and labor intensive than the IHC labeled streptavidin-biotin peroxidase method, we compared the two methods for all samples. Results: The IHC method detected ATL in 67 of the 73 cases (91.8%). Immunolabeled parasites were primarily detected inside macrophages either in the superficial or the deep dermis. Detection was facilitated by the high contrast staining of amastigotes (dark brown) against the light blue background. A lower detection rate (71.2%) was observed with the both of the monoclonal Leishmania antibodies compared to the canine hyperimmune serum. This may have been due to a nonspecific background staining observed in all histological samples rendering positive detection more difficult. The higher efficacy of the canine hyperimmune serum in the IHC method was confirmed by the method using streptavidin-biotin peroxidase as well as that with the polymer-based technology (biotin-avidin-free system). Conclusions: The data are encouraging with regard to validating IHC as a standard alternative method for ATL diagnosis.
URI: http://www.repositorio.ufop.br/handle/123456789/3779
metadata.dc.identifier.doi: https://doi.org/10.1371/journal.pone.0063343
ISSN: 1932-6203
metadata.dc.rights.license: No permission is required from the authors or the publishers. Fonte: Plos <http://www.plos.org/about/open-access/license/>. Acesso em: 08 dez. 2013.
Appears in Collections:DEMSC - Artigos publicados em periódicos

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