Use este identificador para citar ou linkar para este item:
http://www.repositorio.ufop.br/jspui/handle/123456789/12243
Título: | Pharmacokinetic study of xylazine in a zebrafsh water tank, a human‑like surrogate, by liquid chromatography Q‑Orbitrap mass spectrometry. |
Autor(es): | Mato, Rebecca Rodrigues Martucci, Maria Elvira Poleti Anselmo, Carina de Souza Aquino Neto, Francisco Radler de Pereira, Henrique Marcelo Gualberto Sardela, Vinícius Figueiredo |
Palavras-chave: | Metabolism Doping control |
Data do documento: | 2020 |
Referência: | MATO, R. R. et al. Pharmacokinetic study of xylazine in a zebrafsh water tank, a human‑like surrogate, by liquid chromatography Q‑Orbitrap mass spectrometry. Forensic Toxicology, v. 38, p. 108–121, jan. 2020. Disponível em: <https://link.springer.com/article/10.1007/s11419-019-00493-y>. Acesso em: 10 fev. 2020. |
Resumo: | Purpose: This study aims to investigate a zebrafish (Danio Rerio) water tank (ZWT) as an alternative model for the study of the metabolism of xylazine. Methods: The ZWT approach for the study of metabolism consisted of two aquariums, where 18 fish and xylazine were added into the first tank. The second one, with 18 fish without drug, served as a negative control. The samples were submitted to a comprehensive analytical method developed for doping control purposes by liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) operating in five different acquisition modes. Glycoconjugate metabolites were evaluated indirectly by extracting the samples with and without the enzymatic hydrolysis step using β-glucuronidase. Results: In total, 11 phase I and II metabolites were detected and characterized, of which four were previously described for humans and two for horses, and five metabolites were described for the first time. The main metabolites were 4-hydroxylated (M2) and oxygenated (M1) derivatives. Both metabolites were suggested as analytical targets for xylazine misuse. Around 79% of para- and meta-hydroxylated derivatives were in glycoconjugate form, whereas for oxo-hydroxylated and sulfone-hydroxylated derivatives of xylazine, around 83% and 70% were metabolized to the glycoconjugate form, respectively. Conclusions: Xylazine was the subject of extensive metabolism in zebrafish. 4-Hydroxylated (M2) and oxygenated (M1) derivatives were the most abundant phase I metabolites as the main targets for doping control. |
URI: | http://www.repositorio.ufop.br/handle/123456789/12243 |
Link para o artigo: | https://link.springer.com/article/10.1007/s11419-019-00493-y |
DOI: | https://doi.org/10.1007/s11419-019-00493-y |
ISSN: | 1860-8973 |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ARTIGO_PharmacokineticStudyXylazine.pdf Restricted Access | 1,76 MB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.