Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/11082
Title: Paclitaxel-loaded pH-sensitive liposome : new insights on structural and physicochemical characterization.
Authors: Monteiro, Liziane Oliveira Fonseca
Souza, Angelo Malachias de
Lana, Gwenaelle Elza Nathalie Pound
Paniago, Rogerio Magalhães
Mosqueira, Vanessa Carla Furtado
Oliveira, Mônica Cristina de
Barros, André Luís Branco de
Leite, Elaine Amaral
Issue Date: 2018
Citation: MONTEIRO, L. O. F. et al. Paclitaxel-loaded pH-sensitive liposome : new insights on structural and physicochemical characterization. Langmuir, v. 34, n. 20, p. 5728-5737, 2018. Disponível em: <https://pubs.acs.org/doi/10.1021/acs.langmuir.8b00411>. Acesso em: 7 mar. 2019.
Abstract: A long-circulating and pH-sensitive liposome containing paclitaxel (SpHL-PTX) was recently developed by our group. Once in an acidic environment, for example, tumors, these liposomes undergo destabilization, releasing the encapsulated drug. In this way, the aim of this study was to evaluate the molecular and supramolecular interactions between the lipid bilayer and PTX in similar biological environment conditions. High-sensitivity analyses of SpHL-PTX structures were obtained by the small-angle X-ray scattering technique combined with other techniques such as dynamic light scattering, asymmetric flow field-flow fractionation, transmission electron microscopy, and high-performance liquid chromatography. The results showed that PTX incorporation in the liposomal bilayer clearly leads to changes in supramolecular organization of dioleoylphosphatidylethanolamine (DOPE) molecules, inducing the formation of more ordered structures. Changes in supramolecular organization were observed at lower pH, indicating that pH sensitivity was preserved even in the presence of fetal bovine serum proteins. Furthermore, morphological and physicochemical characterization of SpHL-PTX evidenced the formation of nanosized dispersion suitable for intravenous administration. In conclusion, a stable nanosized dispersion of PTX was obtained at pH 7.4 with suitable parameters for intravenous administration. At lower pH conditions, the pH sensitivity of the system was clearly evidenced by changes in the supramolecular organization of DOPE molecules, which is crucial for the delivery of PTX into the cytoplasm of the targeted cells. In this way, the results obtained by different techniques confirm the feasibility of SpHL as a promising tool to PTX delivery in acidic environments, such as tumors.
URI: http://www.repositorio.ufop.br/handle/123456789/11082
metadata.dc.identifier.uri2: https://pubs.acs.org/doi/10.1021/acs.langmuir.8b00411
ISSN: 1520-5827
Appears in Collections:DEFAR - Artigos publicados em periódicos

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