Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/jspui/handle/123456789/10931
Título: Brazilian ironstone plant communities as reservoirs of culturable bacteria with diverse biotechnological potential.
Autor(es): Caneschi, Washington Luiz
Felestrino, Érica Barbosa
Fonseca, Natasha Peixoto
Villa, Morghana Marina
Lemes, Camila Gracyelle de Carvalho
Cordeiro, Isabella Ferreira
Assis, Renata de Almeida Barbosa
Sanchez, Angelica Bianchini
Vieira, Izadora Tabuso
Kamino, Luciana Hiromi Yoshino
Carmo, Flávio Fonseca do
Garcia, Camila Carrião Machado
Moreira, Leandro Marcio
Palavras-chave: Canga
Plasmids
PGPB
Arsenic tolerance
Protection against ROS
Data do documento: 2018
Referência: CANESCHI, W. L. et al. Brazilian ironstone plant communities as reservoirs of culturable bacteria with diverse biotechnological potential. Frontiers in Microbiology, v. 9, p. 1-17, jul. 2018. Disponível em: <https://www.frontiersin.org/articles/10.3389/fmicb.2018.01638/full>. Acesso em: 22 fev. 2019.
Resumo: Extensive mineral extractivism in the Brazilian Iron Quadrangle (IQ) region has destroyed large areas of land, decimating plant species, and their associated microbiota. Very little is known about the microbiota of the region; hence, cultivable bacteria associated with plants of its soils were investigated for their biotechnological potential. Samples were collected from nine plant species and six soils, and 65 cultivable bacterial isolates were obtained. These represent predominantly gram-positive bacilli (70%) capable of producing amylases (55%), proteases (63%), cellulases (47%), indole acetic acid (IAA) (46%), siderophores (26%), and to solubilize phosphate (9%). In addition, 65% of these were resistant to ampicillin, 100% were sensitive to tetracycline, and 97% were tolerant to high arsenic concentrations. Three isolates were studied further: the isolate FOB3 (Rosenbergiella sp.) produced high concentrations of IAA in vitro in the absence of tryptophan – shown by the significant improvement in plant germination and growth rate where the isolate was present. For isolates C25 (Acinetobacter sp.) and FG3 (Serratia sp.), plasmids were purified and inserted into Escherichia coli cells where they modified the physiological profile of the transformed strains. The E. coli::pFG3B strain showed the highest capacity for biofilm production, as well as an increase in the replication rate, arsenic tolerance and catalase activity. Moreover, this strain increased DNA integrity in the presence of arsenic, compared to the wild-type strain. These results help to explain the importance of bacteria in maintaining plant survival in ferruginous, rocky soils, acting as plant growth promoters, and to highlight the biotechnological potential of these bacteria. IMPORTANCE The Iron Quadrangle region is responsible for ∼60% of all Brazilian iron production and, at the same time, is responsible for housing a wide diversity of landscapes, and consequently, a series of endemic plant species and dozens of rare species – all of which have been poorly studied. Studies exploring the microbiota associated with these plant species are limited and in the face of the continuous pressure of extractive action, some species along with their microbiota are being decimated. To understand the potential of this microbiota, we discovered that cultivable bacterial isolates obtained from plants in the ferruginous rocky soil of the Iron Quadrangle region have diverse biotechnological potential, revealing a genetic ancestry still unknown.
URI: http://www.repositorio.ufop.br/handle/123456789/10931
ISSN: 1664302X
Licença: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these term. Fonte: o próprio artigo
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