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Title: Comparing the therapeutic efficacy of different amphotericin Bcarrying delivery systems against visceral leishmaniasis.
Authors: Mendonça, Débora Vasconcelos Costa
Martins, Vivian Tamietti
Lage, Daniela Pagliara
Ribeiro, Patrícia Aparecida Fernandes
Carvalho, Ana Maria Ravena Severino
Dias, Anna Letícia T.
Miyazaki, Carolina Kei
Souza, Daniel Menezes
Roatt, Bruno Mendes
Tavares, Carlos Alberto Pereira
Duarte, Mariana Costa
Coelho, Eduardo Antônio Ferraz
Keywords: Poloxamer 407
Issue Date: 2018
Citation: MENDONÇA, D. V. C. et al. Comparing the therapeutic efficacy of different amphotericin Bcarrying delivery systems against visceral leishmaniasis. Experimental Parasitology, v. 186, p. 24-35, mar. 2018. Disponível em: <>. Acesso em: 22 fev. 2019.
Abstract: Amphotericin B (Amp) has been well-successfully used to treat against Leishmania infection, although high toxicity has been found in patients. In the present study, Amp was administered in Leishmania infantum-infected BALB/c mice by three distinct delivery systems aiming to compare their efficacy against challenge infection, as well as their side effects in a murine visceral leishmaniasis (VL) model. This product was administered in a Poloxamer P407 (Pluronic® F127)-based polymeric micelle system (Amp/M), in the Ambisome® formulation (Lip-Amp) or in a free format (free Amp). Glucantime® (Gluc) was used as a comparative drug. Aiming to evaluate different endpoints of the treatments, the efficacy of the compounds was investigated one and 15-days after the therapeutic regimens, determining the parasite load by a limiting dilution assay and a quantitative PCR (qPCR) technique, as well as evaluating the immune response generated in the infected and treated animals. In the results, Amp/M or Lip-Amp-treated mice presented the best outcomes, since significant parasite load reductions were found in the evaluated organs, as well as a parasite-specific Th1 immune response was observed in the animals. In addition, no hepatic or renal damage was found in these mice. On the other hand, free Amp or Gluc induced toxicity in the animals, which was associated with a low Th1 immune response. Comparatively, Amp/M was the most effective drug in our experimental model, and results showed that the Amp-carrying system could be considered as a future alternative in studies against VL.
ISSN: 00144894
Appears in Collections:DECBI - Artigos publicados em periódicos

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