Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/jspui/handle/123456789/8181
Título: Detailed search for protein kinase(s) involved in plasma membrane H+-ATPase activity regulation of yeast cells.
Autor(es): Pereira, Renata Rebeca
Castanheira, Diogo Dias
Teixeira, Janaina Aparecida
Bouillet, Leoneide Érica Maduro
Ribeiro, Erica Milena de Castro
Trópia, Maria José Magalhães
Alvarez, Florencia
Correa, Lygia Fátima da Mata
Mota, Bruno Eduardo Fernandes
Conceição, Luís Eduardo Fernandes Rodrigues da
Castro, Ieso de Miranda
Brandão, Rogélio Lopes
Palavras-chave: Glucose signaling
Yeast protein kinases
Data do documento: 2015
Referência: PEREIRA, R. R. et al. Detailed search for protein kinase(s) involved in plasma membrane H+-ATPase activity regulation of yeast cells. FEMS Yeast Research, v. 15, p. fov003-fov012, 2015. Disponível em: <https://academic.oup.com/femsyr/article-lookup/doi/10.1093/femsyr/fov003> . Acesso em: 16 jun. 2017.
Resumo: This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sensitivity to hygromycin B. Furthermore, we verified whether the remaining mutants produced enzymes that are appropriately incorporated at plasma membrane. Finally, we measure the kinetic properties of the enzyme in purified plasma membranes from glucose-starved as well as glucose-fermenting cells. We confirmed the kinase Ptk2 involvement in H+−ATPase regulation (increase of affinity for ATP). However, the identification of the kinase(s) responsible for phosphorylation that leads to an increase in Vmax appears to be more complex. Complementary experiments were performed to check how those protein kinases could be related to the control of the plasma membrane H+−ATPase and/or the potential membrane. In summary, our results did not permit us to identify the protein kinase(s) involved in regulating the catalytic efficiency of the plasma membrane H+−ATPase. Therefore, our results indicate that the current regulatory model based on the phosphorylation of two different sites located in the C-terminus tail of the enzyme could be inappropriate.
URI: http://www.repositorio.ufop.br/handle/123456789/8181
Link para o artigo: https://academic.oup.com/femsyr/article-lookup/doi/10.1093/femsyr/fov003
DOI: https://doi.org/10.1093/femsyr/fov003
ISSN: 1567-1364
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