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Title: Iron toxicity mediated by oxidative stress enhances tissue damage in an animal model of diabetes.
Authors: Sampaio, Ana Flávia Santos
Silva, Maísa
Dornas, Waleska Claudia Amaral
Costa, Daniela Caldeira
Silva, Marcelo Eustáquio
Santos, Rinaldo Cardoso dos
Lima, Wanderson Geraldo de
Pedrosa, Maria Lúcia
Keywords: Heart
Reactive oxygen species
Issue Date: 2014
Citation: SAMPAIO, A. F. S. et al. Iron toxicity mediated by oxidative stress enhances tissue damage in an animal model of diabetes. BioMetals, v. 27, p. 349-361, 2014. Disponível em: <>. Acesso em: 19 fev. 2017.
Abstract: Although iron is a first-line pro-oxidant that modulates clinical manifestations of various systemic diseases, including diabetes, the individual tissue damage generated by active oxidant insults has not been demonstrated in current animal models of diabetes. We tested the hypothesis that oxidative stress is involved in the severity of the tissues injury when iron supplementation is administered in a model of type 1 diabetes. Streptozotocin (Stz)-induced diabetic and non-diabetic Fischer ratsweremaintainedwith orwithout a treatment consisting of iron dextran ip at 0.1 mL day-1 doses administered for 4 days at intervals of 5 days. After 3 weeks, an extensive increase (p\0.001) in the production of reactive oxygen species (ROS) in neutrophils of the diabetic animals on iron overload was observed. Histological analysis revealed that this treatment also resulted in higher (p\0.05) tissue iron deposits, a higher (p\0.001) number of inflammatory cells in the pancreas, and apparent cardiac fibrosis, as shown by an increase (p\0.05) in type III collagen levels, which result in dysfunctional myocardial. Carbonyl proteinmodification, amarker of oxidative stress, was consistently higher (p\0.01) in the tissues of the iron-treated rats with diabetes. Moreover, a significant positive correlationwas found betweenROS production andironpancreas stores (r = 0.42, p\0.04), iron heart stores (r = 0.54, p\0.04), and change of the carbonyl protein content in pancreas (r = 0.49, p\0.009), and heart (r = 0.48, p\0.02). A negative correlation was still found between ROS production and total glutathione content in pancreas (r = -0.50, p\0.03) and heart (r = -0.45, p\0.04). In conclusion, our results suggest that amplified toxicity in pancreatic and cardiac tissues in rats with diabetes on iron overload might be attributed to increased oxidative stress.
ISSN: 1572-8773
Appears in Collections:DECBI - Artigos publicados em periódicos

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