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Título: | Antiarrhythmogenic effects of a neurotoxin from the spider Phoneutria nigriventer. |
Autor(es): | Almeida, Alvair Pinto de Andrade, Alexandre Barbosa Ferreira, Anderson José Pires, Andrea Cristina Gomes Damasceno, Dênis Derly Alves, Márcia Netto Magalhães Gomes, Enéas Ricardo de Morais Kushmerick, Christopher Lima, Ricardo de Freitas Prado, Marco Antônio Maximo Prado, Vânia Ferreira Richardson, Michael Cordeiro, Marta do Nascimento Guatimosim, Silvia Gomez, Marcus Vinicius |
Palavras-chave: | Cardiac arrhythmias Isolated heart Atropine Acetylcholine |
Data do documento: | 2011 |
Referência: | ALMEIDA, A. P. de et al. Antiarrhythmogenic effects of a neurotoxin from the spider Phoneutria nigriventer. Toxicon, Oxford, v. 57, p. 217-224, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0041010110004095>. Acesso em: 29 jan. 2017. |
Resumo: | In this study, we evaluated the effects of PhKv, a 4584 Da peptide isolated from the spider Phoneutria nigriventer venom, in the isolated rat heart and in isolated ventricular myocytes. Ventricular arrhythmias were induced by occlusion of the left anterior descending coronary artery for 15 min followed by 30 min of reperfusion. Administration of native PhKv (240 nM) 1 min before or after reperfusion markedly reduced the duration of arrhythmias. This effect was blocked by atropine, thereby indicating the participation of muscarinic receptors in the antiarrhythmogenic effect of PhKv. Notably, recombinant PhKv (240 nM) was also efficient to attenuate the arrhythmias (3.8 0.9 vs. 8.0 1.2 arbitrary units in control group). Furthermore, PhKv induced a significant reduction in heart rate. This bradycardia was partially blunted by atropine and potentiated by pyridostigmine. To further evaluate the participation of acetylcholine on the PhKv effects, we examined the release of this neurotransmitter from neuromuscular junctions. It was found that Phkv (200 nM) significantly increased the release of acetylcholine in this preparation. Moreover, PhKv (250 nM) did not cause any significant change in action potential or Ca2þ transient parameters in isolated cardiomyocytes. Altogether, these findings show an important acetylcholine-mediated antiarrhythmogenic effect of the spider PhKv toxin in isolated hearts. |
URI: | http://www.repositorio.ufop.br/handle/123456789/7487 |
Link para o artigo: | http://www.sciencedirect.com/science/article/pii/S0041010110004095 |
DOI: | https://doi.org/10.1016/j.toxicon.2010.11.013 |
ISSN: | 0041-0101 |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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ARTIGO_AntiarrhyhmogenicEffectsNeurotoxin.pdf Restricted Access | 678,94 kB | Adobe PDF | Visualizar/Abrir |
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