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Título: | Purification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate. |
Autor(es): | Paula, Carmem Aparecida de Sousa, Marcelo Valle de Salgado, Maria Cristina de Oliveira Oliveira, Eduardo Brandt de |
Palavras-chave: | Chymase Somatostatin |
Data do documento: | 1998 |
Referência: | PAULA, C. A. de et al. Purification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate. Biochimica Et Biophysica Acta, v. 1388, p.227-238, 1998. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0167483898001861>. Acesso em: 10 out. 2016. |
Resumo: | A soluble angiotensin (Ang) II-generating enzyme has been purified to homogeneity from the rat mesenteric arterial bed(MAB) perfusate by a combination of gel filtration and affinity chromatographies. The enzyme is a glycoprotein of 28.5 kDa(SDS-PAGE), whose N-terminal sequence is identical with that of the rat pancreatic elastase-2 ; therefore the enzyme willhenceforth be referred to as rat MAB elastase-2. When Ang I was used as the substrate, the enzyme specifically released AngII and the dipeptide His-Leu (Km=36WM;Kcat= 1530 min31). The catalytic efficiency (Kcat/Km= 42.5 min31WM31) of thisreaction was comparable to those of other known Ang I-converting enzymes. The proteolytic specificity of the purifiedenzyme toward mellitin, oxidized insulin B chain, somatostatin-14 and renin substrate tetradecapeptide suggested that theenzyme-substrate interaction was defined by an extended substrate binding site, typical of elastases-2 of pancreatic origin. According to the sensitivity of the rat MAB elastase-2 to various inhibitors this enzyme could be described as a member ofthe chymostatin-sensitive group of Ang II-forming serine proteases. The localization and biochemical properties of thisenzyme suggest that it might play a role in the regional control of vascular tonus. |
URI: | http://www.repositorio.ufop.br/handle/123456789/7306 |
Link para o artigo: | http://www.sciencedirect.com/science/article/pii/S0167483898001861 |
DOI: | https://doi.org/10.1016/S0167-4838(98)00186-1 |
ISSN: | 1570-9639 |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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ARTIGO_PurificationSubstrateSpecificity.pdf Restricted Access | 322,09 kB | Adobe PDF | Visualizar/Abrir |
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