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Título: | Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni. |
Autor(es): | Reis, Eneida Virgínia Pereira, Roberta Verciano Gomes, Matheus de Souza Passos, Liana Konovaloff Jannotti Babá, Élio Hideo Coelho, Paulo Marcos Zech Mattos, Ana Carolina Alves de Couto, Flávia Fernanda Búbula Borges, William de Castro Cota, Renata Guerra de Sá |
Palavras-chave: | Vault complex Resistance Differential expression Schistosoma mansoni |
Data do documento: | 2014 |
Referência: | REIS, E. V. et al. Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni. Parasitology International, v. 1, p. 1-10, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576913001657>. Acesso em: 08 nov. 2014. |
Resumo: | Vaults are ribonucleoproteins (13MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The exact function of this complex is unknown, although the biological role of vaults has been associated with multidrug resistance phenotypes and signal transduction pathways. Genomic analysis showed that model organisms, such as Caenorhabditis elegans and Drosophila melanogaster, do not possess genes encoding vaults. However, we have found that vault-related genes are present in the Schistosoma mansoni genome. These observations raised questions on the involvement of vaults in mechanisms of adaptation of the parasite in its mammalian host. Therefore, molecular characterisation of the putative Major Vault Protein performed using bioinformatics tools showed that this vault component is highly conserved in S. mansoni. The MVP expression level was quantified by qRT-PCR using total RNA from susceptible (LE) and resistant (LE-PZQ) adult worm lineages, cercariae and mechanically transformed schistosomula(MTS) cultured for 3.5, 24, 48 and 72h in vitro. Our results suggest a stage-specific expression in all developmental stages analysed. Western blotting has shown upregulation of SmMVP in the MTS-3.5, 72 h and resistant adult worms, and similar levels in all other stages. Furthermore, SmMVPwas found differentially expressed in adult males and females fromthe susceptible lineage. Further studies should clarify whether SmMVP is somehow linked to drug resistance in S. mansoni. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4551 |
DOI: | https://doi.org/10.1016/j.parint.2013.10.005 |
ISSN: | 1383-5769 |
Licença: | O periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120114254. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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