Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/3964
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dc.contributor.authorChiari, Egler-
dc.contributor.authorDias, João Carlos Pinto-
dc.contributor.authorLana, Marta de-
dc.contributor.authorChiari, Cléa de Andrade-
dc.date.accessioned2014-11-19T15:39:43Z-
dc.date.available2014-11-19T15:39:43Z-
dc.date.issued1989-
dc.identifier.citationA Revista da Sociedade Brasileira de Medicina Tropical permite o depósito das versões pré-print e pós-print de um artigo. Permite remixagem, adaptação e nova criação a partir da obra para fins não comerciais desde que seja atribuído o crédito ao autor (CC BY-NC). Fonte: Diadorim <http://diadorim.ibict.br/handle/1/127>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.issn0037-8682-
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/3964-
dc.description.abstractWith the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas’ disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15’). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X^ = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.pt_BR
dc.language.isoen_USpt_BR
dc.subjectChagas' diseasept_BR
dc.subjectParasitological diagnosispt_BR
dc.subjectHemoculture and xenodiagnosispt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.titleHemocultures for the parasitological diagnosis of human chronic Chagas' disease.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseA Revista da Sociedade Brasileira de Medicina Tropical permite o depósito das versões pré-print e pós-print de um artigo. Permite remixagem, adaptação e nova criação a partir da obra para fins não comerciais desde que seja atribuído o crédito ao autor (CC BY-NC). Fonte: Diadorim <http://diadorim.ibict.br/handle/1/127>. Acesso em: 10 fev. 2020.pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S0037-86821989000100004-
Appears in Collections:DEACL - Artigos publicados em periódicos

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