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Title: Relationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modifed nanocapsules.
Authors: Mosqueira, Vanessa Carla Furtado
Legrand, Philippe
Gulik, Annette
Bourdon, Olivier
Gref, Ruxandra
Labarre, Denis
Barratt, Gillian
Keywords: Nanocapsules
Poly(D,L-lactide-co-ethylene oxide) copolymers
Complement activation
Cellular uptake
Physicochemical characterization
Issue Date: 2001
Citation: MOSQUEIRA, V. C. F. et al. Relationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modifed nanocapsules. Biomaterials, Guildford, v. 22, n. 22, p. 2967-2979, 2001. Disponível em: <>. Acesso em: 20 ago. 2014.
Abstract: The aim of our work was to examine the relationship between modi"cations of the surface of nanocapsules (NC) by adsorption or covalent grafting of poly(ethylene oxide) (PEG), and changes in their phospholipid (PL) content on complement activation (C3 cleavage) and on uptake by macrophages. The physicochemical characterization of the NC included an investigation of their properties, such as surface charge, size, hydrophilicity, morphology and homogeneity. This is the "rst time that such properties have been correlated with biological interactions for NC, a novel carrier system with a structure more complex than nanospheres. C3 crossed immunoelectrophoresis revealed the reduced activation for NC with longer PEG chain and higher density, although all formulations induced C3 cleavage to a lesser or greater extent. NC bearing PEG covalently bound to the surface were weaker activators of complement than plain PLA [poly(D,L-lactide)] NC or nanospheres (NS). Furthermore, the #uorescent/confocal microscopy of J774A1 cells in contact with NC reveal a dramatically reduced interaction with PEG-bearing NC. However, the way in which PEG was attached (covalent or adsorbed) seemed to a!ect the mechanism of uptake. Taken together, these results suggest that the low level of protein binding to NC covered with a high density of 20 kDa PEG chains is likely to be due to the steric barriers surrounding these particles, which prevents protein adsorption and reduces their interaction with macrophages. 2001 Elsevier Science Ltd. All rights reserved.
ISSN: 0142-9612
metadata.dc.rights.license: O periódico Biomaterials concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460320954961.
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