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Title: Buccal micronucleus cytome assay : inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil.
Authors: Rohr, Paula
Silva, Gabrieli Flesch da
Vicentini, Veronica Elisa Pimenta
Almeida, Igor Vivian de
Santos, Raquel Alves dos
Takahashi, Catarina Satie
Goulart, Mirian Oliveira
Silva, Glenda Nicioli da
Oliveira, Luiza Barbosa de
Grisolia, Cesar Koppe
Piau, Tathyana Benetis
Branco, Carmen Lucia Bassi
Reis, Érica de Melo
Galvão, Marcos Felipe de Oliveira
Medeiros, Silvia Regina Batistuzzo de
Monteiro, Magaly Sales
Lopes, Reynaldo Assis de Vasconcelos
Brandão, Sabrina Fuziger Inácio
Batista, Nelson Jorge Carvalho
Paz, Márcia Fernanda Correia Jardim
Alencar, Juliana da Silva Araújo
Keywords: DNA damage
Exfoliated cells
Issue Date: 2020
Citation: ROHR, P. et al. Buccal micronucleus cytome assay: Inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil. Toxicology Letters, v. 333, p. 242–250, out. 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: The Buccal Micronucleus Cytome Assay (BMCyt) has become an important biomonitoring tool for assessing cytogenetic damage in many studied populations. Each laboratory applies protocols that vary according to the method of collecting and preparing samples. Besides, Brazil is a country of great territorial extensions that received immigrants from various parts of the world with different genetic backgrounds. Therefore, the present study aimed to evaluate the inter-laboratory variation in scoring the same set of slides using the more comprehensive scoring criteria, to standardize the BMCyt protocol, to observe the basal alterations in populations of different Brazilian regions and to compare it with other places around the world. Our results showed that a valuable number of laboratories participated, ten laboratories from different regions of the country, for the validation of the BMCyt in human biomonitoring studies, resulting in the 804 healthy individuals. This was possible because we observed: a range of measures needs to be considered, such as the baseline frequency of DNA damage and cell death in non-exposed individuals; age when grouped showed an influence on DNA damage, although when evaluated by group we did not see an influence; association between smoking habit and all endpoints of the BMCyt (except karyolytic cells) was evident; the basal MN frequency, in the majority of groups, follows those around the world; and the BMCyt was confirmed as a good health status biomarker. We emphasize the need for constant discussions on the parameters of cell death due to greater difficulty among the analyzers.
ISSN: 0378-4274
Appears in Collections:DEACL - Artigos publicados em periódicos

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