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Título: | Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection. |
Autor(es): | Ribeiro, Patrícia Aparecida Fernandes Dias, Daniel Silva Lage, Daniela Pagliara Martins, Vivian Tamietti Costa, Lourena Emanuele Santos, Thaís Teodoro de Oliveira Ramos, Fernanda Fonseca Tavares, Grasiele de Sousa Vieira Mendonça, Débora Vasconcelos Costa Ribeiro, Fernanda Ludolf Gomes, Dawidson Assis Rodrigues, Michele Angela Chávez Fumagalli, Miguel Angel Silva, Eduardo Sergio da Galdino, Alexsandro Sobreira Duarte, Mariana Costa Roatt, Bruno Mendes Souza, Daniel Menezes Teixeira Junior, Antonio Lucio Coelho, Eduardo Antônio Ferraz |
Palavras-chave: | Immune response |
Data do documento: | 2019 |
Referência: | RIBEIRO, P. A. F. et al. Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection. Molecular Immunology, v. 106, p. 108-118, fev. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub>. Acesso em: 10 fev. 2020. |
Resumo: | Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. The immunity elicited by both vaccination regimens reduced the parasitism in liver, spleen, bone marrow and draining lymph nodes, being associated with high levels of IFN-γ, IL-12, GM-CSF, and specific IgG2a antibody, besides low production of IL-4, IL-10, and protein and parasite-specific IgG1 antibodies. CD4+ T cells contributed more significantly to IFN-γ production in the rLiHyP/saponin group, while CD8+ T cells were more important in the production of this cytokine in the DNA LiHyP group. In addition, increased IFN-γ secretion, along with low levels of IL-10, were found when PBMCs from treated VL subject and healthy individuals were stimulated with the recombinant protein. In conclusion, when administered either as a DNA plasmid or recombinant protein, LiHyP can direct the immune response towards a Th1 immune profile, protecting animals against L. infantum infection; therefore, it can be seen as a promising immunogen against human VL. |
URI: | http://www.repositorio.ufop.br/handle/123456789/12001 |
Link para o artigo: | https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub |
DOI: | https://doi.org/10.1016/j.molimm.2018.12.025 |
ISSN: | 0161-5890 |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_ImmunogenicityProtectiveEfficacy.pdf Restricted Access | 1,69 MB | Adobe PDF | Visualizar/Abrir |
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