Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/jspui/handle/123456789/12001
Título: Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection.
Autor(es): Ribeiro, Patrícia Aparecida Fernandes
Dias, Daniel Silva
Lage, Daniela Pagliara
Martins, Vivian Tamietti
Costa, Lourena Emanuele
Santos, Thaís Teodoro de Oliveira
Ramos, Fernanda Fonseca
Tavares, Grasiele de Sousa Vieira
Mendonça, Débora Vasconcelos Costa
Ribeiro, Fernanda Ludolf
Gomes, Dawidson Assis
Rodrigues, Michele Angela
Chávez Fumagalli, Miguel Angel
Silva, Eduardo Sergio da
Galdino, Alexsandro Sobreira
Duarte, Mariana Costa
Roatt, Bruno Mendes
Souza, Daniel Menezes
Teixeira Junior, Antonio Lucio
Coelho, Eduardo Antônio Ferraz
Palavras-chave: Immune response
Data do documento: 2019
Referência: RIBEIRO, P. A. F. et al. Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection. Molecular Immunology, v. 106, p. 108-118, fev. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub>. Acesso em: 10 fev. 2020.
Resumo: Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. The immunity elicited by both vaccination regimens reduced the parasitism in liver, spleen, bone marrow and draining lymph nodes, being associated with high levels of IFN-γ, IL-12, GM-CSF, and specific IgG2a antibody, besides low production of IL-4, IL-10, and protein and parasite-specific IgG1 antibodies. CD4+ T cells contributed more significantly to IFN-γ production in the rLiHyP/saponin group, while CD8+ T cells were more important in the production of this cytokine in the DNA LiHyP group. In addition, increased IFN-γ secretion, along with low levels of IL-10, were found when PBMCs from treated VL subject and healthy individuals were stimulated with the recombinant protein. In conclusion, when administered either as a DNA plasmid or recombinant protein, LiHyP can direct the immune response towards a Th1 immune profile, protecting animals against L. infantum infection; therefore, it can be seen as a promising immunogen against human VL.
URI: http://www.repositorio.ufop.br/handle/123456789/12001
Link para o artigo: https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub
DOI: https://doi.org/10.1016/j.molimm.2018.12.025
ISSN: 0161-5890
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