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Título: | Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae. |
Autor(es): | Pereira, Michele B. P. Tisi, Renata Fietto, Luciano Gomes Cardoso, Anamaria de Souza França, Mônica M. Carvalho, Fernanda Machado de Trópia, Maria José Magalhães Martegani, Enzo Castro, Ieso de Miranda Brandão, Rogélio Lopes |
Palavras-chave: | Depolarizing compounds Saccharomyces cerevisiae |
Data do documento: | 2008 |
Referência: | PEREIRA, M. B. P. et al. Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae. FEMS Yeast Research, v. 8, n.4, p.622-630, jun. 2008. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00380.x/pdf>. Acesso em: 18 jul. 2012. |
Resumo: | The plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase Cdependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H1-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase. |
URI: | http://www.repositorio.ufop.br/handle/123456789/1164 |
ISSN: | 15671364 |
Licença: | O Periódico FEMS Yeast Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500810576. |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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