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Title: Physical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni.
Authors: Valentim, Cláudia Laignier Lage
Gomes, Matheus de Souza
Jeremias, Wander de Jesus
Cunha, Juliana Cecília
Oliveira, Guilherme Corrêa de
Botelho, Ana Cristina de Carvalho
Pimenta, Paulo Filemon Paolucci
Passos, Liana Konovaloff Jannotti
Cota, Renata Guerra de Sá
Babá, Élio Hideo
Issue Date: 2008
Citation: VALENTIM, C. L. L. et al. Physical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni. The Journal of Parasitology, v. 94, p. 993-994, 2008. Disponível em: <>. Acesso em: 20 mar. 2017.
Abstract: Schistosoma mansoni is 1 of the causative agents of schistosomiasis, an endemic disease in 76 countries of the world. The study of its genome, estimated to be 270 Mb, is very important to understanding schistosome biology, the mechanisms of drug resistance, and immune evasion. Repetitive elements constitute more than 40% of the S. mansoni genome and may play a role in the parasite evolution. The retrotransposons Boudicca, a long terminal repeat (LTR), and Perere 03, a non-LTR, are present in a high number in the S. mansoni genome and were localized with the use of fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS). Bacterial artificial chromosomes (BAC) clones containing the retrotransposons Boudicca and Perere 03 were selected by bioinformatic analysis and used as probes in FISH. Using metaphase chromosomes from sporocysts and the FISH and PRINS techniques, we were able to map these retrotransposons. Perere 03 was localized in the euchromatic regions of the short arm of chromosome 2 and Boudicca in
ISSN: 1937-2345
Appears in Collections:DECBI - Artigos publicados em periódicos

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