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dc.contributor.authorCristiano, Maykon Passos-
dc.contributor.authorSimões, Talitta Guimarães-
dc.contributor.authorLopes, Denilce Meneses-
dc.contributor.authorPompolo, Silvia das Graças-
dc.date.accessioned2015-05-28T16:58:24Z-
dc.date.available2015-05-28T16:58:24Z-
dc.date.issued2014-
dc.identifier.citationCRISTIANO. M. P. et al. Cytogenetics of Melitoma segmentaria (Fabricius, 1804) (Hymenoptera, Apidae) reveals differences in the characteristics of heterochromatin in bees. Comparative Cytogenetics, v. 8, p. 223-231, 2014. Disponível em: <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205491/>. Acesso em: 20 mai. 2015.pt_BR
dc.identifier.issn1993-0771-
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/5559-
dc.description.abstractTo date, more than 65 species of Brazilian bees (of the superfamily Apoidea) have been cytogenetically studied, but only a few solitary species have been analyzed. One example is the genus Melitoma Lepele¬tier & Serville, 1828, for which there is no report in the literature with regard to cytogenetic studies. The objective of the present study is to analyze the chromosome number and morphology of the species Melitoma segmentaria (Fabricius, 1804), as well as to determine the pattern of heterochromatin dis¬tribution and identify the adenine–thymine (AT)- and guanine–cytosine (GC)-rich regions. Melitoma segmentaria presents chromosome numbers of 2n=30 (females) and n=15 (males). With C-banding, it is possible to classify the chromosomes into seven pseudo-acrocentric pairs (AM), seven pseudo-acrocentric pairs with interstitial heterochromatin (AMi), and one totally heterochromatic metacentric pair (Mh). Fluo¬rochrome staining has revealed that heterochromatin present in the chromosomal arms is rich in GC base pairs (CMA3+) and the centromeric region is rich in AT base pairs (DAPI+). The composition found for Melitoma diverges from the pattern observed in other bees, in which the heterochromatin is usually rich in AT. In bees, few heterochromatic regions are rich in GC and these are usually associated with or localized close to the nucleolus organizer regions (NORs). Silver nitrate impregnation marks the heterochromatin present in the chromosome arms, which makes identification of the NOR in the chromosomes impos¬sible. As this technique reveals proteins in the NOR, the observation that is made in the present study suggests that the proteins found in the heterochromatin are qualitatively similar to those in the NOR.pt_BR
dc.language.isoen_USpt_BR
dc.subjectCytogenetic characterizationpt_BR
dc.subjectHeterochromatinpt_BR
dc.subjectFluorochromespt_BR
dc.subjectSolitary beespt_BR
dc.subjectKaryotype evolutionpt_BR
dc.titleCytogenetics of Melitoma segmentaria (Fabricius, 1804) (Hymenoptera, Apidae) reveals differences in the characteristics of heterochromatin in bees.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseOs trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.3897/compcytogen.v8i3.7510-
Appears in Collections:DEBIO - Artigos publicados em periódicos

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