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Title: Preliminary analysis of miRNA pathway in Schistosoma mansoni.
Authors: Gomes, Matheus de Souza
Cabral, Fernanda Janku
Passos, Liana Konovaloff Jannotti
Carvalho, Omar
Rodrigues, Vanderlei
Babá, Élio Hideo
Cota, Renata Guerra de Sá
Keywords: Schistosoma mansoni
Gene expression
Post-transcriptional gene silencing
Issue Date: 2009
Citation: GOMES, M. de S. et al. Preliminary analysis of miRNA pathway in Schistosoma mansoni. Parasitology International, v. 58, p. 61-68, 2009. Disponível em: <>. Acesso em: 08 nov. 2014.
Abstract: RNA silencing refers to a series of nuclear and cytoplasmatic processes involved in the post-transcriptional regulation of gene expression or post-transcriptional gene silencing (PTGS), either by sequence-specific mRNA degradation or by translational arrest. The best characterized small RNAs are microRNAs (miRNAs), which predominantly perform gene silencing through post-transcriptional mechanisms. In this work we used bioinformatic approaches to identify the parasitic trematode Schistosoma mansoni sequences that are similar to enzymes involved in the post-transcriptional gene silencing mediated by miRNA pathway.We used amino acid sequences of well-known proteins involved in the miRNA pathway against S. mansoni genome and transcriptome databases identifying a total of 13 putative proteins in the parasite. In addition, the transcript levels of SmDicer1 and SmAgo2/3/4 were identified by qRT-PCR using cercariae, adult worms, eggs and in vitro cultivated schistosomula. Our results showed that the SmDicer1 and SmAgo2/3/4 are differentially expressed during schistosomula development, suggesting that the miRNA pathway is regulated at the transcript level and therefore may control gene expression during the life cycle of S. mansoni.
ISSN: 1383-5769
metadata.dc.rights.license: O periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120424363.
Appears in Collections:DECBI - Artigos publicados em periódicos

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