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Título: | Schistosoma mansoni : functional proteasomes are required for development in the vertebrate host. |
Autor(es): | Cota, Renata Guerra de Sá Borges, William de Castro Evangelista, Elísio Alberto Kettelhut, Isis do Carmo Rodrigues, Vanderlei |
Palavras-chave: | Schistosoma mansoni Parasites |
Data do documento: | 2005 |
Referência: | SÁ, R. G. et al. Schistosoma mansoni: functional proteasomes are required for development in the vertebrate host. Experimental Parasitology, v. 109, n. 4, p. 228-236, 2005. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0014489405000093>. Acesso em: 08 nov. 2014. |
Resumo: | Proteasomes are multi-subunit proteases involved in several mechanisms and thought to contribute to the regulation of cellular homeostasis. Here, we report for the Wrst time biochemical evidence for the existence of a ubiquitin–proteasome proteolytic pathway in this parasite. Proteasomes from both cercariae and adult worms exhibited a high preference for hydrolysis of the substrate Suc- LLVY-AMC, although in the cercariae extract the rate of hydrolysis was 50% lower when compared to adult worms extracts. The same diVerence in proteasome activities was observed when endogenous proteins were broken down in the presence of ATP and ubiquitin. Additionally, accumulation of high molecular weight conjugates was observed when cercariae were pre-incubated with proteasome inhibitors. Finally, we present evidence that during experimental schistosomiasis, proteasome inhibitors were able to reduce the number of lung stage schistosomula, reduce the worm burden and consequently decrease the egg output in infected mice. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4284 |
DOI: | https://doi.org/10.1016/j.exppara.2005.01.002 |
ISSN: | 0014-4894 |
Licença: | O periódico Experimental Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3521411366469. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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ARTIGO_SchistosoMamansoniFunctional.pdf | 325,15 kB | Adobe PDF | Visualizar/Abrir |
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