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http://www.repositorio.ufop.br/jspui/handle/123456789/17842
Título: | In vitro and in vivo acute toxicity of a novel citrate-coated magnetite nanoparticle. |
Autor(es): | Rocha, José Marcos Vieira Souza, Valeria Barbosa de Panunto, Patricia Costa Nicolosi, Jacqueline Spacagna Silva, Emanueli do Nascimento da Cadore, Solange Londono, Oscar Moscoso Muraca, Diego Tancredi, Pablo Brot, Marina de Nadruz, Wilson Ruiz, Ana Lucia Tasca Gois Knobel, Marcelo Schenka, André Almeida |
Palavras-chave: | Alanine aminotransferase Alkaline phosphatase |
Data do documento: | 2022 |
Referência: | ROCHA, J. M. V. et al. In vitro and in vivo acute toxicity of a novel citrate-coated magnetite nanoparticle. PLoS One, v. 17, artigo e0277396, 2022. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0277396>. Acesso em: 01 ago. 2023. |
Resumo: | Magnetic nanoparticles (MNps) have become powerful tools for multiple biomedical applications such as hyperthermia drivers, magnetic resonance imaging (MRI) vectors, as well as drug-delivery systems. However, their toxic effects on human health have not yet been fully elucidated, especially in view of their great diversity of surface modifications and functionalizations. Citrate-coating of MNps often results in increased hydrophilicity, which may positively impact their performance as drug-delivery systems. Nonetheless, the consequences on the intrinsic toxicity of such MNps are unpredictable. Herein, novel magnetite (Fe3O4) nanoparticles covered with citrate were synthesized and their potential intrinsic acute toxic effects were investigated using in vitro and in vivo models. The proposed synthetic pathway turned out to be simple, quick, inexpensive, and reproducible. Concerning toxicity risk assessment, these citrate-coated iron oxide nanoparticles (IONps) did not affect the in vitro viability of different cell lines (HaCaT and HepG2). Moreover, the in vivo acute dose assay (OECD test guideline #425) showed no alterations in clinical parameters, relevant biochemical variables, or morphological aspects of vital organs (such as brain, liver, lung and kidney). Iron concentrations were slightly increased in the liver, as shown by Graphite Furnace Atomic Absorption Spectrometry and Perls Prussian Blue Staining assays, but this finding was considered non-adverse, given the absence of accompanying functional/clinical repercussions. In conclusion, this study reports on the development of a simple, fast and reproducible method to obtain citrate-coated IONps with promising safety features, which may be used as a drug nanodelivery system in the short run. |
URI: | http://www.repositorio.ufop.br/jspui/handle/123456789/17842 |
DOI: | https://doi.org/10.1371/journal.pone.0277396 |
ISSN: | 1932-6203 |
Licença: | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: PDF do artigo. |
Aparece nas coleções: | DEQUI - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_VitroVivoAcute.pdf | 4,06 MB | Adobe PDF | Visualizar/Abrir |
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