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Título: | A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum. |
Autor(es): | Machado, Amanda Sanchez Lage, Daniela Pagliara Vale, Danniele Luciana Freitas, Camila Simões de Linhares, Flávia Prata Cardoso, Jamille Mirelle de Oliveira Pereira, Isabela Amorim Gonçalves Ramos, Fernanda Fonseca Tavares, Grasiele de Sousa Vieira Ribeiro, Fernanda Ludolf Silva, João Augusto Oliveira da Bandeira, Raquel Soares Simoes, Aratti Cãndido Duarte, Mariana Costa Oliveira, Jamil Silvano de Christodoulides, Myron Chávez Fumagalli, Miguel Angel Roatt, Bruno Mendes Martins, Vivian Tamietti Coelho, Eduardo Antônio Ferraz |
Palavras-chave: | Polymeric micelles Saponin Hypothetical protein Amastigote Visceral leishmaniasis |
Data do documento: | 2022 |
Referência: | MACHADO, A. S. et al. A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum. Acta tropica, v. 230, artigo 106412, jun. 2022. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X22001103?via%3Dihub>. Acesso em: 11 out. 2022. |
Resumo: | Vaccination against visceral leishmaniasis (VL) should be considered as a control measure to protect against disease, and amastigote-specific proteins could help to develop such vaccines, since this parasite form is in contact with the host immune system during the active disease. In this study, a Leishmania amastigote-specific protein, LiHyG, was evaluated as recombinant protein (rLiHyG) as vaccine candidate against Leishmania infan- tum infection in BALB/c mice. The protein was associated with saponin (rLiHyG/Sap) or Poloxamer 407-based polymeric micelles (rLiHyG/Mic) as adjuvants, and animals receiving saline, saponin or micelle as controls. Immunological and parasitological analyses were performed before (n = 8 per group; as primary endpoint) and after (n = 8 per group; as secondary endpoint) infection. Results showed that, in both endpoints, rLiHyG/Sap and rLiHyG/Mic induced higher levels of IFN-γ, IL-12 and GM-CSF in spleen cell cultures from vaccinated animals, besides elevated presence of IgG2a isotype antibodies. Decreased hepatotoxicity and ‘positive lymphoprolifer- ative response were also found after challenge. Such findings reflected in significantly lower levels of parasite load found in their spleens, livers, bone marrows and draining lymph nodes. In conclusion, rLiHyG associated with Th1-type adjuvant could be considered for future studies as vaccine candidate to protect against VL. |
URI: | http://www.repositorio.ufop.br/jspui/handle/123456789/16407 |
Link para o artigo: | https://www.sciencedirect.com/science/article/pii/S0001706X22001103?via%3Dihub |
ISSN: | 0001-706X |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_RecombinantLeishmaniaAmastigota.pdf Restricted Access | 1,69 MB | Adobe PDF | Visualizar/Abrir |
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