Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/jspui/handle/123456789/13827
Título: Evaluation of the protective efficacy of a Leishmania protein associated with distinct adjuvants against visceral leishmaniasis and in vitro immunogenicity in human cells.
Autor(es): Ribeiro, Patrícia Aparecida Fernandes
Dias, Daniel Silva
Lage, Daniela Pagliara
Mendonça, Débora Vasconcelos Costa
Vale, Danniele Luciana
Ramos, Fernanda Fonseca
Carvalho, Lívia Mendes
Carvalho, Ana Maria Ravena Severino
Steiner, Bethina Trevisol
Roque, Marjorie Coimbra
Silva, João Augusto Oliveira da
Oliveira, Jamil Silvano de
Tavares, Grasiele de Sousa Vieira
Martins, Vivian Tamietti
Chávez Fumagalli, Miguel Angel
Roatt, Bruno Mendes
Moreira, Ricardo Luiz Fontes
Souza, Daniel Menezes
Duarte, Mariana Costa
Oliveira, Mônica Cristina de
Ávila, Ricardo Andrez Machado de
Teixeira Junior, Antonio Lucio
Coelho, Eduardo Antônio Ferraz
Palavras-chave: Hypothetical proteins
Liposome
Saponin
Peripheral blood mononuclear cells
Immunogenicity
Data do documento: 2020
Referência: RIBEIRO, P. A. F. et al. Evaluation of the protective efficacy of a Leishmania protein associated with distinct adjuvants against visceral leishmaniasis and in vitro immunogenicity in human cells. Parasitology Research, v. 119, p. 2609-2622, jun. 2020. Disponível em: <https://link.springer.com/article/10.1007/s00436-020-06752-x>. Acesso em: 10 jun. 2021.
Resumo: The treatment against visceral leishmaniasis (VL) presents problems, mainly related to the toxicity and/or high cost of the drugs. In this context, a prophylactic vaccination is urgently required. In the present study, a Leishmania protein called LiHyE, which was suggested recently as an antigenic marker for canine and human VL, was evaluated regarding its immunogenicity and protective efficacy in BALB/c mice against Leishmania infantum infection. In addition, the protein was used to stimulate peripheral blood mononuclear cells (PBMCs) from VL patients before and after treatment, as well as from healthy subjects. Vaccination results showed that the recombinant (rLiHyE) protein associated with liposome or saponin induced effective protection in the mice, since significant reductions in the parasite load in spleen, liver, draining lymph nodes, and bone marrow were found. The parasitological protection was associated with Th1-type cell response, since high IFN-γ, IL-12, and GM-CSF levels, in addition to low IL-4 and IL-10 production, were found. Liposome induced a better parasitological and immunological protection than did saponin. Experiments using PBMCs showed rLiHyE-stimulated lymphoproliferation in treated patients’ and healthy subjects’ cells, as well as high IFN-γ levels in the cell supernatant. In conclusion, rLiHyE could be considered for future studies as a vaccine candidate against VL..
URI: http://www.repositorio.ufop.br/jspui/handle/123456789/13827
Link para o artigo: https://link.springer.com/article/10.1007/s00436-020-06752-x
DOI: https://doi.org/10.1007/s00436-020-06752-x
ISSN: 1432-1955
Aparece nas coleções:DECBI - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
ARTIGO_EvaluationProtectionEfficacy.pdf
  Restricted Access
1,66 MBAdobe PDFVisualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.