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|Title:||Quantitative proteomics of enriched esophageal and gut tissues from the human blood fluke Schistosoma mansoni pinpoints secreted proteins for vaccine development.|
|Authors:||Neves, Leandro Xavier|
Wilson, R. Alan
Harman, Victoria Margaret Elizabeth
Beynon, Robert J.
Eyers, Claire E.
Borges, William de Castro
|Citation:||NEVES, L. X. et al. Quantitative proteomics of enriched esophageal and gut tissues from the human blood fluke Schistosoma mansoni pinpoints secreted proteins for vaccine development. Journal of Proteome Research, v. 19, p. 314-326, fev. 2020. Disponível em: <https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00531>. Acesso em: 10 fev. 2020.|
|Abstract:||Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine development. Rhesus macaques can selfcure following schistosome infection, generating antibodies that target proteins from the tegument, gut, and esophagus, the last of which is the least investigated. We developed a dissection technique that permitted increased sensitivity in a comparative proteomics profiling of schistosome esophagus and gut. Proteome analysis of the male schistosome esophagus identified 13 proteins encoded by microexon genes (MEGs), 11 of which were uniquely located in the esophageal glands. Based on this and transcriptome information, a QconCAT was designed for the absolute quantification of selected targets. MEGs 12, 4.2, and 4.1 and venom allergen-like protein 7 were the most abundant, spanning over 245 million to 6 million copies per cell, while aspartyl protease, palmitoyl thioesterase, and galactosyl transferase were present at <1 million copies. Antigenic variation by alternative splicing of MEG proteins was confirmed together with a specialized machinery for protein glycosylation/secretion in the esophagus. Moreover, some gastrodermal secretions were highly enriched in the gut, while others were more uniformly distributed throughout the parasite, potentially indicating lysosomal activity. Collectively, our findings provide a more rational, better-oriented selection of schistosome vaccine candidates in the context of a proven model of protective immunity.|
|Appears in Collections:||DECBI - Artigos publicados em periódicos|
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