Please use this identifier to cite or link to this item:
Title: Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice.
Authors: Moreira, Thais Garcias
Santos, Ana Cristina Gomes
Horta, Laila Sampaio
Miranda, Mariana Camila Gonçalves
Santiago, Andrezza Fernanda
Gonçalves, Juliana Lauar
Reis, Daniela Silva dos
Castro Junior, Archimedes Barbosa de
Santos, Luísa Lemos dos
Guimarães, Mauro Andrade de Freitas
Aguilar, Edenil Costa
Pap, Attila
Amaral, Joana Ferreira do
Leite, Jacqueline Isaura Alvarez
Machado, Denise Carmona Cara
Rezende, Rafael Machado
Nagy, Laszlo
Faria, Ana Maria Caetano de
Maioli, Tatiani Uceli
Keywords: Colitis
Issue Date: 2018
Citation: MOREIRA, T. G. et al. Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice. The Journal of Nutritional Biochemistry, v. 57, p. 238-245, jul. 2018. Disponível em: <>. Acesso em: 7 mar. 2019.
Abstract: Dietary supplementation with conjugated linoleic acid (CLA) has been proposed for weight management and to prevent gut inflammation. However, some animal studies suggest that supplementation with CLA leads to the development of nonalcoholic fatty liver disease. The aims of this study were to test the efficiency of CLA in preventing dextran sulfate sodium (DSS)-induced colitis, to analyze the effects of CLA in the liver function, and to access putative liver alterations upon CLA supplementation during colitis. So, C57BL/6 mice were supplemented for 3 weeks with either control diet (AIN-G) or 1% CLA-supplemented diet. CLA content in the diet and in the liver of mice fed CLA containing diet were accessed by gas chromatography. On the first day of the third week of dietary treatment, mice received ad libitum a 1.5%–2.5% DSS solution for 7 days. Disease activity index score was evaluated; colon and liver samples were stained by hematoxylin and eosin for histopathology analysis and lamina propria cells were extracted to access the profile of innate cell infiltrate. Metabolic alterations before and after colitis induction were accessed by an open calorimetric circuit. Serum glucose, cholesterol, triglycerides and alanine aminotransaminase were measured; the content of fat in liver and feces was also accessed. CLA prevented weight loss, histopathologic and macroscopic signs of colitis, and inflammatory infiltration. Mice fed CLA-supplemented without colitis induction diet developed steatosis, which was prevented in mice with colitis probably due to the higher lipid consumption as energy during gut inflammation. This result suggests that CLA is safe for use during gut inflammation but not at steady-state conditions.
ISSN:  0955-2863
Appears in Collections:DENCS - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
  Restricted Access
535,5 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.