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Título: | Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish infected from vaccinated dogs. |
Autor(es): | Andrade, Renata Aline de Reis, Alexandre Barbosa Gontijo, Célia Maria Ferreira Braga, Lidiane Bento Rocha, Roberta Dias Rodrigues Araújo, Márcio Sobreira Silva Vianna, Leonardo Rocha Martins Filho, Olindo Assis |
Palavras-chave: | Leishmania chagasi Flow cytometry Canine visceral leishmaniasis Vaccine |
Data do documento: | 2007 |
Referência: | ANDRADE, R. A. de et al. Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish infected from vaccinated dogs. Veterinary Immunology and Immunopathology, v. 116, n. 1-2, p. 85-97, mar. 2007. Disponível em: <https://www.sciencedirect.com/science/article/pii/S016524270700027X>. Acesso em: 10 de jul. 2012. |
Resumo: | Leishmune® vaccination covers a broader number of endemic areas of canine visceral leishmaniasis (CVL) and therefore the development of new serological devices able to discriminate CVL from Leishmune® vaccinees becomes an urgent need considering the post-vaccine seroconversion detected throughout conventional methodologies. Herein, we have described the establishment of a flow cytometry based methodology to detect anti-fixedL. ( L. ) chagasipromastigotes antibodies (FC-AFPA-IgG, FC-AFPA-IgG1 and FC-AFPA-IgG2) in sera samples from Leishmania ( Leishmania ) chagasiinfected dogs and Leishmune® vaccinees. The results of FC-AFPA were reported along the sera titration curve (1:128–1:524,288), as percentage-of-positive-fluorescent-parasite (PPFP). The use of PPFP = 20% as a cut-off edge to segregate negative and positive results at sera dilution 1:2048 revealed outstanding performance indexes that elect FC-AFPA-IgG and IgG2 (both detected by polyclonal FITC-labeled second step reagent) applicable to the serological diagnosis of CVL, with 100% of specificity for both IgG and IgG2 and 97 and 93% of sensitivity, respectively. Moreover, FC-AFPA-IgG, applied at sera dilution 1:2048, also appeared as a useful tool to discriminate L. chagasiinfected dogs from Leishmune® vaccinees, with 76% of specificity. Outstanding likelihood indexes further support the performance of FC-AFPA-IgG for exclusion diagnosis of CVL in Leishmune® vaccinees. Analysis of FC-AFPA-IgG at sera dilution 1:8192 revealed the most outstanding indexes, demonstrating that besides the ability of PPFP 20% to exclude the diagnosis of CVL, a PPFP values higher 80%, mostly observed for infected dogs (INF) have a minimal change to come from a non-infected animal (NI) or Leishmune®. |
URI: | http://www.repositorio.ufop.br/handle/123456789/1032 |
ISSN: | 01652427 |
Licença: | O periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3291410040834. |
Aparece nas coleções: | DEACL - Artigos publicados em periódicos |
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